Compositions and methods for optimized hybridization using modified solutions
AssigneeAgilent Technologies, Inc.
InventorsTheodore R. Sana, Paul K. Wolber, Clotilde S. Perbost
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Abstract
The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH2)C═O, where R is amino or alkyl. A method for use of the chemical component and composition is also disclosed.
Claims21
A composition that allows a probe and target to hybridize at a temperature lower than their standard hybridization temperature, comprising a chemical component of the formula:
- R(NH2)C═O
- where R is an amino or alkyl group having up to about 50 carbon atoms.
A method that allows a probe and target to hybridize at a temperature lower than their standard hybridization temperature, comprising:
- (a) adding to the probe and target a chemical component of the formula:
- R(NH2)C═O
- where R is an amino or an alkyl group having up to about 50 carbon atoms;
- (b) heating the probe and target in the presence of the added component;
- and
- (c) allowing the probe and target to hybridize.
A kit for hybridizing biopolymers at temperatures below their standard hybridization temperatures, comprising:
- (a) a micro array;
- (b) a composition for use with said micro array;
- and
- (c) a target for detection.
A composition that allows a probe on a micro array surface to hybridize to a target at a temperature lower than their standard hybridization temperature, comprising a chemical component of the formula:
- R(NH2)C═O
- where R is an amino or alkyl group having up to about 50 carbon atoms.
A method that allows a probe on a micro array surface to hybridize to a target at a temperature lower than their standard hybridization temperature, comprising:
- (a) adding to the probe and target a chemical component of the formula:
- R(NH2)C═O where R is an amino or an alkyl group;
- (b) heating the probe and target in the presence of the added component;
- and
- (c) allowing the probe and target to hybridize.
Dependencies are inferred from the claim text.