Complementary DNAs encoding proteins with signal peptides
AssigneeSerono Genetics Institute S.A.
InventorsJean-Baptiste Dumas Milne Edwards, Lydie Bougueleret, Severin Jobert
Life of this patent
Estimating the term…
Abstract
The sequences of cDNAs encoding secreted proteins are disclosed. The cDNAs can be used to express secreted proteins or fragments thereof or to obtain antibodies capable of specifically binding to the secreted proteins. The cDNAs may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. The cDNAs may also be used to design expression vectors and secretion vectors.
Claims30
A purified or isolated nucleic acid comprising the sequence of one of SEQ ID NOs:
- 24-73 or a sequence complementary thereto.
A purified or isolated nucleic acid comprising at least 12 consecutive bases of the sequence of one of SEQ ID NOs:
- 24-73 or one of the sequences complementary thereto.
A purified or isolated nucleic acid comprising the full coding sequences of one of SEQ ID NOs:
- 24-73, wherein the full coding sequence comprises the sequence encoding signal peptide and the sequence encoding mature protein.
A purified or isolated nucleic acid comprising the nucleotides of one of SEQ ID NOs:
- 24-73 which encode a mature protein.
A purified or isolated nucleic acid comprising the nucleotides of one of SEQ ID NOs:
- 24-73 which encode the signal peptide.
A purified or isolated nucleic acid encoding a polypeptide having the sequence of one of the sequences of SEQ ID NOs:
- 74-123.
A purified or isolated nucleic acid encoding a polypeptide having the sequence of a mature protein included in one of the sequences of SEQ ID NOs:
- 74-123.
A purified or isolated nucleic acid encoding a polypeptide having the sequence of a signal peptide included in one of the sequences of SEQ ID NOs:
- 74-123.
A purified or isolated protein comprising the sequence of one of SEQ ID NOs:
- 74-123.
A purified or isolated polypeptide comprising at least 10 consecutive amino acids of one of the sequences of SEQ ID NOs:
- 74-123.
An isolated or purified polypeptide comprising a signal peptide of one of the polypeptides of SEQ ID NOs:
- 74-123.
An isolated or purified polypeptide comprising a mature protein of one of the polypeptides of SEQ ID NOs:
- 74-123.
A method of making a protein comprising one of the sequences of SEQ ID NO:
- 74-123, comprising the steps of:
- obtaining a cDNA comprising one of the sequences of sequence of SEQ ID NO:
- 24-73;
- inserting said cDNA in an expression vector such that said cDNA is operably linked to a promoter;
- and
- introducing said expression vector into a host cell whereby said host cell produces the protein encoded by said cDNA.
A purified or isolated antibody capable of specifically binding to a protein having the sequence of one of SEQ ID NOs:
- 74-123.
In an array of polynucleotides of at least 15 nucleotides in length, the improvement comprising inclusion in said array of at least one of the sequences of SEQ ID NOs:
- 24-73, or one of the sequences complementary to the sequences of SEQ ID NOs:
- 24-73, or a fragment thereof of at least 15 consecutive nucleotides.
A purified or isolated nucleic acid of at least 15 bases capable of hybridizing under stringent conditions to the sequence of one of SEQ ID NOs:
- 24-73 or a sequence complementary to one of the sequences of SEQ ID NOs:
- 24-73.
A purified or isolated antibody capable of binding to a polypeptide comprising at least 10 consecutive amino acids of the sequence of one of SEQ ID NOs:
- 74-123.
A computer readable medium having stored thereon a sequence selected from the group consisting of a cDNA code of SEQID NOs. 24-73 and a polypeptide code of SEQ ID NOs. 74-123.
A computer system comprising a processor and a data storage device wherein said data storage device has stored thereon a sequence selected from the group consisting of a cDNA code of SEQID NOs. 24-73 and a polypeptide code of SEQ ID NOs. 74-123.
A method for comparing a first sequence to a reference sequence wherein said first sequence is selected from the group consisting of a cDNA code of SEQID NOs. 24-73 and a polypeptide code of SEQ ID NOs. 74-123 comprising the steps of:
- reading said first sequence and said reference sequence through use of a computer program which compares sequences;
- and
- determining differences between said first sequence and said reference sequence with said computer program.
A method for identifying a feature in a sequence selected from the group consisting of a cDNA code of SEQID NOs. 24-73 and a polypeptide code of SEQ ID NOs. 74-123 comprising the steps of:
- reading said sequence through the use of a computer program which identifies features in sequences;
- and
- identifying features in said sequence with said computer program.
A purified or isolated nucleic acid comprising a contiguous span of at least 12 nucleotides of the sequence of one of SEQ ID NOs:
- 24-73 or one of the sequences complementary thereto, wherein said contiguous span comprises at least 1 of the nucleotide positions of polynucleotides described in Table III.
A purified or isolated nucleic acid comprising a contiguous span of at least 12 nucleotides of the sequence of one of the polynucleotides described in Table III or one of the sequences complementary thereto.
Dependencies are inferred from the claim text.