Compositions, methods and kits for determining the presence of Mycoplasma genitalium in a test sample
AssigneeGen-Probe Incorporated
InventorsMelissa M. Cunningham, James P. Light II
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Abstract
The present invention relates to oligonucleotides useful for determining the presence of Mycoplasma pneumoniae and/or Mycoplasma genitalium in a test sample. The oligonucleotides of the present invention may be incorporated into hybridization assay probes, capture probes and amplification primers, and used in various combinations thereof.
Claims27
A hybridization assay probe for use in determining the presence of Mycoplasma genitalium in a test sample, said probe comprising a first oligonucleotide having a first target binding region, wherein the base sequence of said first target binding region consists of or is contained within a base sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8,
- wherein said first target binding region is capable of forming a detectable hybrid with nucleic acid derived from Mycoplasma genitalium under stringent hybridization conditions,
- wherein said probe is not capable of forming a detectable hybrid with nucleic acid derived from Mycoplasma pneumoniae under said conditions, and
- wherein said probe does not comprise another base sequence region overlapping with or in addition to said first target binding region which is capable of forming a stable hybrid with nucleic acid derived from Mycoplasma genitalium under said conditions.
Dependencies are inferred from the claim text.