Analytical devices based on diffusion boundary layer calibration and quantitative sorption
OwnerJanusz B. Pawliszyninventor
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Abstract
A method and device for determining the concentration of analytes in a sample. The device has a surface with an extraction coating thereon. The method is carried out while the sample is highly agitated to maintain a substantially constant boundary layer. The sample is brought into contact with the coating for a limited time so that all the analytes that pass through the boundary layer are adsorbed by the coating. The amount of analytes in the coating is determined and the concentration of those same analytes in the sample is then calculated from the diffusion coefficient of the analytes. The device is first calibrated using analytes of known concentration.
Claims38
A method of determining the concentration of analytes of interest in a sample using a solid phase microextraction device having a surface containing an extraction coating, said method comprising bringing said sample into contact with said coating while highly agitating said sample under controlled conditions to maintain a substantially constant boundary layer between said sample and said coating, limiting a time of contact between said sample and said coating so that all analytes that pass through said boundary layer are adsorbed, terminating said contact and determining the amount of each analyte of interest in said coating and calculating the concentration of the analytes of interest in said sample by using the diffusion coefficient for said analytes and said amount in said coating.
A method of determining the concentration of analytes of interest in a sample using an extraction device having a membrane, said method comprising bringing said sample into contact with said membrane for a sufficient time to allow microextraction to occur, said membrane having a large surface area so that all analytes that contact said membrane can be adsorbed, limiting the contact time between the sample and the membrane, separating the membrane from the sample, determining the amount of each analyte of interest in said membrane and calculating the concentration of said analytes of interest in said sample using the diffusion coefficient for the analytes and said amount in said membrane.
A method of calibrating a device for determining the concentration of analytes of interest in a sample, said device being an extraction device and having at least one of a surface containing an extraction coating and a membrane therein, said method comprising bringing said sample into contact with at least one of said coating and said membrane while highly agitating said sample under controlled conditions to maintain a substantially constant boundary layer between said sample and at least one of said coating and said membrane, limiting a time of contact between said sample and at least one of said coating and said membrane so that all analytes that have passed through said boundary layer are adsorbed by at least one of said coating and said membrane, terminating said contact and determining the amount of each analyte of interest in at least one of said coating and said membrane, measuring the concentration of analytes of interest in said sample or starting with a known concentration of analytes of interest in a sample, comparing the amount of each analyte of interest in at least one of said coating and said membrane with the known concentration to calibrate the device and calibrating the device based on the diffusion coefficient for the analytes of interest.
A device for determining the concentration of analytes of interest in a sample, said device comprising a solid phase microextraction device having a surface containing an extraction coating, agitation means to highly agitate the sample under controlled conditions during microextraction, said coating being sized and shaped to fit into an injection port of an analytical instrument.
Dependencies are inferred from the claim text.