Plant regulatory sequences for selective control of gene expression
OwnerTimothy W. Conner, Patrice Dubois +2inventors
InventorsTimothy W. Conner, Patrice Dubois, Marianne Malven, James D. Masucci
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Abstract
Promoters from male reproductive tissues were isolated from corn (Zea mays). These promoters can be used in plants to regulate transcription of target genes including genes for control of fertility, insect or pathogen tolerance, herbicide tolerance or any gene of interest.
Claims27
An isolated nucleic acid comprising a sequence selected from the group consisting of SEQ ID NOS:79-98, or a fragment, region, or cis element of said sequence thereof, said isolated nucleic acid being capable of regulating transcription of an operably linked DNA sequence.
A promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 79-98 and fragments thereof.
A cell comprising a DNA construct comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region, or cis element of said sequence thereof, and operably linked to said nucleic acid sequence, a transcribable DNA sequence and a 3′ non-translated region.
A transgenic plant comprising a DNA construct comprising an isolated nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region, or cis element of said sequence thereof, and operably linked to said nucleic acid sequence, a transcribable DNA sequence and a 3′ non-translated region.
A method of regulating transcription of a DNA sequence comprising operably linking the DNA sequence to a promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98.
A method of making a transgenic plant comprising introducing into a cell of a plant a DNA construct comprising:
- (i) a promoter comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:79-98 or a fragment, region or cis element thereof, and, operably linked to the promoter, (ii) a transcribable DNA sequence and (iii) a 3′ non-translated region.
A method of isolating at least two 5′ regulatory sequences that confer enhanced expression of operably linked genes in male reproductive tissues from a plant comprising:
- (i) evaluating a collection of nucleic acid sequences of ESTs derived from at least one cDNA library prepared from a plant cell type of interest;
- (ii) comparing EST sequences from at least one target plant cDNA library and at least one non-target cDNA libraries of ESTs from a different plant cell type;
- (iii) subtracting common EST sequences found in both target and non-target libraries;
- (iv) designing gene specific primers from the remaining EST sequences after said subtraction;
- and
- (v) isolating the corresponding 5′ flanking and regulatory sequences from a genomic library prepared from the target plant comprising the use of said primers.
Dependencies are inferred from the claim text.