Starch modification
OwnerMichael Meyrick Burrell, Manash Chatterjeeinventors
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Abstract
The present invention relates to a method of altering starch synthesis in a plant by modifying the starch priming activity of the plant. In particular, this is achieved by altering the expression or activity of a starch primer which is preferably encoded by the sequence of SEQ ID NO: 1 or a sequence substantially homologous thereto. Also provided are plants in which the starch priming activity has been altered and plant parts.
Claims45
An isolated nucleic acid molecule that:
- (i) comprises a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 3, or a fragment thereof;
- (ii) comprises a nucleotide sequence at least 40% identical to SEQ ID NOs:
- 1 or 2, or a complement thereof as determined using the BESTFIT or GAP programs with a gap weight of 50 and a length weight of 3;
- or
- (iii) hybridizes to a nucleic acid molecule consisting of SEQ ID NOs:
- 1 or 2 under conditions of hybridization comprising washing at 60° C. twice for 15 minutes in 2×SSC, 0.5% SDS or a complement thereof.
An isolated nucleic acid molecule that:
- (i) comprises a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 11, or a fragment thereof;
- (ii) comprises a nucleotide sequence at least 70% identical to SEQ ID NO:
- 10, or a complement thereof as determined using the BESTFIT or GAP programs with a gap weight of 50 and a length weight of 3, wherein the nucleotide sequence does not encode the amino acid sequence set forth in SEQ ID NO:
- 35;
- or
- (iii) hybridizes to a nucleic acid molecule consisting of SEQ ID NO:
- 10 under stringent conditions of hybridization, or a complement thereof, wherein the nucleotide sequence does not encode the amino acid sequence set forth in SEQ ID NO:
- 35.
An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence that is at least 98% identical to SEQ ID NO:
- 9.
An isolated nucleic acid molecule thereof comprising the nucleotide sequence of SEQ ID NO:
- 8, or a complement thereof.
An isolated nucleic acid molecule that:
- (i) comprises a nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NOs:
- 7, 13, 15, 17, 19, 21, 22, 24, 26, 28, 30, 32, 34, or a fragment thereof,
- (ii) comprises a nucleotide sequence at least 70% identical to SEQ ID NOs:
- 4, 5, 6, 12, 14, 16, 18, 20, 23, 25, 27, 29, 31, 33, or a complement thereof as determined using the BESTFIT or GAP programs with a gap weight of 12 and a length weight of 4;
- or
- (iii) hybridizes to a nucleic acid molecule consisting of SEQ ID NOs:
- 4, 5, 6, 12, 14, 16, 18, 20, 23, 25, 27, 29, 31, 33 under stringent conditions of hybridization, or a complement thereof.
An isolated polypeptide comprising the amino acid sequence of amino acid residues 1-65 of SEQ ID NO:
- 3, or a fragment thereof.
An isolated polypeptide comprising:
- (i) an amino acid sequence that is at least 98% identical to SEQ ID NO:
- 9 as determined using the BESTFIT or GAP programs with a gap weight of 12 and a length weight of 4;
- (iii) an amino acid sequence encoded by the nucleic acid molecule of SEQ ID NO:
- 8, or a complement thereof;
- or (v) an amino acid sequence of SEQ ID NO:
- 9, or a fragment thereof.
A fragment of a polypeptide comprising at least 8 amino acid residues, wherein said fragment is a portion of the polypeptide encoded by a nucleic acid molecule selected from the group consisting of exon I, exon II, exon III, exon IV and exon V of SEQ ID NO:
- 1.
A polypeptide comprising the amino acid sequence of SEQ ID:
- 3, 7, 9, 11, 13, 15, 17, 19, 21, 22, 24, 26, 28, 30, 32, 34 which further comprises one or more conservative amino acid substitution.
A cell comprising a 35S CaMV constitutive promoter operably linked to a nucleic acid molecule of SEQ ID NO:2, or a rice actin promoter operably linked to a RNA interference construct comprising fragments of a nucleic acid molecule of SEQ ID NO:2, wherein said rice actin promoter confers expression of said fragments.
Dependencies are inferred from the claim text.