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US 2003/0200560 A1Published application
Herbicide resistant plants
AssigneeSyngenta Limited
InventorsSimon Anthony James Warner, Timothy Robert Hawkes, Christopher John Andrews
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AssigneeSyngenta Limited
InventorsSimon Anthony James Warner, Timothy Robert Hawkes, Christopher John Andrews
Estimating the term…
The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic Oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene.
A glyphosate resistant EPSPS enzyme wherein in comparison with the wild type EPSPS the protein sequence is modified in that a first position is mutated so that the residue at this position is lie rather than Thr and a second position is mutated so that the residue at this position is Ser rather than Pro, the mutations being introduced into EPSPS sequences which comprise the conserved region GNAGTAMRPL in the wild type enzyme such that the modified sequence reads GNAGIAMRSL, wherein the enzyme does not comprise the sequence K(AN)AKRAVVVGCGGKFPVE, characterised in that the enzyme is stable and possesses at least one of the following sequence motifs (i) to (viii) in which X is any amino acid and Z, Z1 and Z2 are any amino acid other than those specified:
A polynucleotide encoding an EPSPS, which polynucleotide is obtainable by screening plant genomic DNA libraries with a polynucleotide constituting an intron located within SEQ ID Nos. 4 or 5.
A polynucleotide according to the preceding claim, wherein the CTP and PPDK encoding sequences are non-heterologous with respect to each other.
A polynucleotide according to the preceding claim, wherein the said highly expressed gene is selected from the group consisting of those which encode glucanase, chalcone synthase and Rubisco.
A polynucleotide according to the preceding claim wherein the herbicide is a protoporphyrinogen oxidase (PPGO) inhibitor, in particular butefenicil, and the corresponding resistance protein is a PPGO or inhibitor resistant variant thereof.
A polynucleotide according to any preceding claim, which is modified in that mRNA instability motifs and/or unwanted splice regions are removed, or crop preferred codons are used so that expression of the thus modified polynucleotide in a plant yields substantially similar protein having a substantially similar activity/function to that obtained by expression of the protein encoding regions of the unmodified polynucleotide in the organism in which they are endogenous.
A polynucleotide according to the preceding claim, wherein the degree of identity between the modified polynucleotide and a polynucleotide endogenously contained within the said plant and encoding substantially the same protein is such as to prevent co-suppression between the modified and endogenous sequences.
A polynucleotide according to the preceding claim, wherein the said degree is less than about 70%.
A vector comprising the polynucleotide of any preceding claim.
Plants according to the preceding claim, wherein the said capable protein is a fusion of a CTP and PPS or a fusion of a CTP and a PPDK.
A method according to the preceding claim, further comprising application to the field either before or after application of the glyphosate herbicide of one or more of the following:
A method according to the preceding claim, wherein the transformation involves the introduction of the polynucleotide into the material by:
A method according to the preceding claim, wherein the transformed material is selected by its resistance to glyphosate.
A method according to the preceding claim, wherein the biological material is of plant origin.
A method according to the preceding claim, wherein the plant is a dicot.
A method according to'the preceding claim, wherein the dicot is selected from group consisting of soybean, cotton, sugar beet, Brassica napus, campestris or oleraceae.
A method according to the preceding claim, wherein the plant is a dicot selected from the group consisting soybean, cotton, sugar beet, Brassica napus, catizpestiris or oleraceae.
Dependencies are inferred from the claim text.