Enhanced proteins and methods for their use
AssigneeMonsanto Company
InventorsWilliam D. Rapp, Jiexin Peng, Gautham Nadig, Tyamagondlu Venkatesh
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Abstract
The present invention generally relates to the field of plant genetics and protein biochemistry. More specifically, the present invention relates to modified proteins having an increased number of essential amino acids. The invention provides proteins modified to have an increased number of essential amino acids, nucleic acid sequences encoding the enhanced proteins, and methods of designing, producing, and using the same. The invention also includes compositions, transformed host cells, transgenic plants and seeds containing the enhanced proteins, and methods for preparing and using the same.
Claims20
A modified polypeptide comprising a substitution of 1 or more essential amino acids selected from the group consisting of isoleucine, lysine, methionine, threonine and tryptophan into an unmodified polypeptide having an amino acid sequence of SEQ ID NO:
- 1, wherein said modified polypeptide is capable of accumulating in a seed.
A modified β-conglycinin polypeptide comprising a substitution of 1 or more essential amino acids selected from the group consisting of threonine, isoleucine, tryptophan, valine, arginine, lysine, methionine, and histidine into an unmodified β-conglycinin polypeptide having an amino acid sequence of SEQ ID NO:
- 1, wherein said modified β-conglycinin polypeptide is capable of accumulating in a seed.
A recombinant nucleic acid molecule encoding a modified β-conglycinin polypeptide comprising a substitution of 1 or more essential amino acids selected from the group consisting of threonine, isoleucine, tryptophan, valine, arginine, lysine, methionine, and histidine into an unmodified β-conglycinin polypeptide having an amino acid sequence of SEQ ID NO:
- 1, wherein said modified β-conglycinin polypeptide is capable of accumulating in a cell.
Dependencies are inferred from the claim text.