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US 2003/0199682 A1Published application
Novel plant expression constructs
AssigneeMonsanto Technology LLC
InventorsKaren L. Fincher, Stanislaw Flasinski, Jack Q. Wilkinson
Life of this patent
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Abstract
The present invention relates to novel plant expression constructs. More specifically the present invention provides DNA constructs comprising 5′ regulatory sequences for modulating the expression of operably linked genes in plants.
Claims50
A DNA construct comprising:
- an expression cassette comprising a promoter DNA sequence comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- a structural DNA sequence that encodes an agronomically useful protein;
- and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter DNA sequence is heterologous with respect to the structural DNA sequence.
1 more element
A chimeric promoter DNA sequence comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- and said at least one cis element is operably linked to a heterologous promoter sequence.
A DNA construct comprising:
- and expression cassette comprising a chimeric promoter DNA sequence comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- and said at least one cis element is operably linked to a heterologous promoter sequence;
- a structural DNA sequence;
- and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter DNA sequence is heterologous with respect to the structural DNA sequence.
2 more elements
A DNA construct comprising a chimeric promoter DNA sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30;
- a structural DNA sequence;
- and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the chimeric promoter and the 3′ non-translated region, and the chimeric promoter DNA sequence is heterologous with respect to the structural DNA sequence.
A DNA construct comprising a first expression cassette and a second expression cassette, wherein said first expression cassette comprises a promoter DNA sequence comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- a structural DNA sequence that encodes an agronomically useful protein;
- and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter DNA sequence is heterologous with respect to the structural DNA sequence;
- and said second expression cassette comprises a promoter DNA sequence comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:1, SEQ ID NO:12, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- a structural DNA sequence that encodes an agronomically useful protein;
- and a 3′ non-translated region that functions in plants to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter DNA sequence is heterologous with respect to the structural DNA sequence.
4 more elements
A method of expressing a structural DNA sequence in a plant, the method comprising:
- (1) providing a DNA construct comprising a promoter that is functional in a plant cell, the promoter comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12;, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- a structural DNA sequence encoding an agronomically useful protein;
- and a 3′ non-translated region that functions to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter is heterologous with respect to the structural DNA sequence;
- (2) introducing the DNA construct into a plant cell;
- and (3) regenerating the plant cell to produce the plant such that the structural DNA sequence is expressible in the plant.
3 more elements
A method of expressing a structural DNA sequence in a plant, the method comprising:
- (1) providing a DNA construct comprising a promoter that is functional in a plant cell, the promoter comprising a sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30;
- a structural DNA sequence;
- and a 3′ non-translated region that functions to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the structural gene is operably linked to the promoter and the 3′ non-translated region, and the promoter is heterologous with respect to the structural DNA sequence;
- (2) introducing the DNA construct into a plant cell;
- and (3) regenerating the plant cell to produce the plant such that the structural DNA sequence is expressible in the plant.
3 more elements
A method of controlling weeds, the method comprising:
- (1) providing a crop plant transformed with a DNA construct that comprises a promoter that is functional in a plant cell, the promoter comprising at least one cis element derived from a sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, and SEQ ID NO:12;, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
- a glyphosate tolerance gene;
- and a 3′ non-translated region that functions to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the glyphosate tolerance gene is operably linked to the promoter and the 3′ non-translated region, and the promoter is heterologous with respect to the glyphosate tolerance gene;
- and (2) applying to the crop plant a sufficient amount of glyphosate to control weeds without damaging the crop plant.
2 more elements
A method of controlling weeds, the method comprising:
- (1) providing a crop plant transformed with a DNA construct that comprises a promoter that is functional in a plant cell, the promoter comprising a sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30;
- a glyphosate tolerance gene;
- and a 3′ non-translated region that functions to cause the addition of polyadenylated nucleotides to the 3′ end of the RNA sequence;
- wherein the glyphosate tolerance gene is operably linked to the promoter and the 3′ non-translated region, and the promoter is heterologous with respect to the glyphosate tolerance gene;
- and (2) applying to the crop plant a sufficient amount of glyphosate to control weeds without damaging the crop plant.
2 more elements
Dependencies are inferred from the claim text.