Amino acid sequences facilitating penetration of a substance of interest into cells and/or cell nuclei
AssigneeCellectis
InventorsEustrate Avrameas, Therese Ternynck
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Abstract
The invention concerns an amino acid sequence capable of facilitating penetration of a substance of interest into cells and/or cell nuclei, characterized in that it is capable of reacting in vivo with aminoglycans. Optionally the sequence is derived from a protein of human origin.
Claims66
A peptide comprising an amino acid sequence selected from the group consisting of:
- a) (XBBBXXBX)n;
- b) (XBBXBX)n;
- a) (BBXmBBXo)n;
- b) (XBBXXBX)n;
- and
- c) (BXBB)n
- wherein
- B is a basic amino acid;
- X is a non-basic amino acid;
- each m is independently an integer from zero to five;
- each n is independently an integer between one and ten;
- each o is independently an integer between zero to five,
- wherein the peptide translocates across a biological membrane.
A peptide comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1-48, wherein the peptide translocates across a biological membrane.
A peptide comprising at least two or more domains, wherein one domain comprises an amino acid sequence selected from the group consisting of:
- a) XBBBXXBX;
- b) XBBXBX;
- c) BBXmBBXo;
- d) XBBXXBX;
- and
- e) BXBB,
- wherein
- B is a basic amino acid;
- X is a non-basic amino acid;
- each m is independently an integer from zero to five;
- each o is independently an integer between zero to five, and
- wherein a second domain comprises an amino acid sequence selected from the group consisting of:
- a) XBBBXXBX;
- b) XBBXBX;
- c) BBXmBBXo;
- d) XBBXXBX;
- e) BXBB;
- and
- f) an antibody fragment,
- wherein
- B is a basic amino acid;
- X is a non-basic amino acid;
- each m is independently an integer from zero to five;
- each o is independently an integer between zero to five, and
- wherein the peptide translocates across a biological membrane.
A peptide comprising at least two domains, wherein each of the domains comprises an amino acid sequence selected from the group consisting of:
- a) a CDR3 region of an anti-DNA antibody;
- and
- b) a CDR2 region of an anti-DNA antibody;
- wherein the peptide translocates across a biological membrane into the cytoplasm of a cell.
A peptide comprising an amino acid sequence capable of translocating across a biological membrane, wherein the peptide is a) derived from a lipoprotein, b) comprises at least 4 basic amino acids, and c) interacts with aminoglycans.
Dependencies are inferred from the claim text.