Combination of bryostatin and paclitaxel for treating cancer
AssigneeSloan-Kettering Institute for Cancer Research
InventorsGary K. Schwartz, Anthony P. Albino
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Abstract
This invention provides methods for screening a modulating agent which when combined with antitumor therapeutic agent increases apoptosis in tumor cells. This invention also provides methods for screening antitumor therapeutic agents suitable for combination therapy with a protein kinase C inhibitors capable of potentiating apoptosis in tumor cells. This invention further provides different combination therapies comprising the specific protein kinase C inhibitors and the antitumor therapeutic agents.
Claims35
A method for screening for a modulating agent which when combined with an antitumor therapeutic agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with an amount of the modulating agent and with an antitumor therapeutic agent known to cause apotosis when used in combination with an appropriate modulating agent in an amount effective to increases apoptosis in tumor cells;
- (b) determining the degree of apoptosis of tumor cells from step (a);
- and
- (c) comparing the degree of apoptosis determined in step (b) with the degree of apoptosis of tumor cells which are treated with only the antitumor therapeutic agent or only the modulating agent, an increase in apoptosis indicating that the modulating agent in combination with an antitumor therapeutic agent is capable of increasing apoptosis in tumor cells.
A method for screening for a modulating agent which when combined with an antitumor therapeutic agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with an amount of the modulating agent for a period of time;
- (b) contacting the tumor cells from step (a) with an antitumor therapeutic agent known to increase apotosis when used in combination with a modulating agent in an amount effective to increase apoptosis;
- (c) determining the degree of apoptosis of tumor cells from step (b);
- and
- (d) comparing the degree of apoptosis determined in step (b) with the degree of apoptosis of tumor cells which are treated with only the modulating agent, or only the antitumor therapeutic agent, an increase in apoptosis indicating that the modulating agent in combination with an antitumor therapeutic agent is capable of increasing apoptosis in tumor cells.
A method for screening for a modulating agent which when combined with an antitumor therapeutic agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with an antitumor therapeutic agent known to increases apoptosis in tumor cells when used in combination with an appropriate modulating agent in an amount effective to increase apoptosis for a period of time;
- (b) contacting the tumor cells from step (a) with an amount of the modulating agent;
- (c) determining the degree of apoptosis of tumor cells from step (b);
- and
- (d) comparing the degree of apoptosis determined in step (b) with the degree of apoptosis of tumor cells which are treated with only the antitumor therapeutic agent or only the modulating agent, an increase in apoptosis indicating that the modulating agent in combination with an antitumor therapeutic agent is capable of increasing apoptosis in tumor cells.
A method for screening for an antitumor therapeutic agent which when combined with a modulating agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with a modulating agent known to increase apoptosis in tumor cells when used in combination with an appropriate antitumor therapeutic agent in an amount effective to increases apoptosis in tumor cells and with an amount of the antitumor therapeutic agent;
- (b) determining the degree of apoptosis of tumor cells from step (a);
- and
- (c) determining the degree of apoptosis of tumor cells from step (b);
- and
- (d) comparing the degree of apoptosis determined in step (c) with the degree of apoptosis of tumor cells which are treated with only the modulating agent or only the antitumor therapeutic agent, an increase in apoptosis indicating that the antitumor therapeutic agent in combination with the modulating agent is capable of increasing apoptosis in tumor cells.
A method for screening for an antitumor therapeutic agent which when combined with a modulating agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with a modulating agent known to increase apoptosis in tumor cells when used in combination with an appropriate antitumor therapeutic agent in an amount effective to increases apoptosis in tumor cells for a period of time;
- (b) contacting the tumor cells from step (a) with an amount of the antitumor therapeutic agent;
- (c) determining the degree of apoptosis of tumor cells from step (b);
- and
- (d) comparing the degree of apoptosis determined in step (c) with the degree of apoptosis of tumor cells which are treated with only the modulating agent or only the antitumor therapeutic agent, an increase in apoptosis indicating that the antitumor therapeutic agent in combination with the modulating agent is capable of increasing apoptosis in tumor cells.
A method for screening for an antitumor therapeutic agent which when combined with a modulating agent increases apoptosis in tumor cells comprising the steps of:
- (a) contacting tumor cells with an amount of the antitumor therapeutic agent for a period time;
- (b) contacting the tumor cells from step (a) with a modulating agent known to increase apoptosis in tumor cells when used in combination with an appropriate antitumor therapeutic agent in an amount effective to increases apoptosis in tumor cells;
- (c) determining the degree of apoptosis of tumor cells from step (b);
- and
- (d) comparing the degree of apoptosis determined in step (c) with the degree of apoptosis of tumor cells which are treated with only the modulating agent or only the antitumor therapeutic agent, an increase in apoptosis indicating that the antitumor therapeutic agent in combination with the modulating agent is capable of increasing apoptosis in tumor cells.
A method for treating cancer in a subject comprising administering to the subject an effective amount of at least one antitumor therapeutic agent, and at least one modulating agent, sequentially or concomitantly.
A method for increasing apoptosis in tumor cells comprising contacting tumor cells with an effective amount of a modulating agent capable of increasing apoptosis during, prior to, or after treatment with one or more antitumor therapeutic agents.
Dependencies are inferred from the claim text.