Methods and compositions for regulating cellular signaling
OwnerGabriel Nunez, Naohiro Inohara +1inventors
InventorsGabriel Nunez, Naohiro Inohara, Akihiro Muto
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Abstract
The present invention related to methods and compositions for modulating cellular signaling. In particular, the present invention relates to PKK and RICK3 proteins. The present invention further relates the to use of PKK and RICK3 proteins in modulating NF-κB signaling. The present invention thus provides novel targets for drug screening and therapeutics.
Claims34
An isolated and purified nucleic acid comprising a sequence encoding a protein selected from the group consisting of SEQ ID NOs:
- 3 and sequences that are at least 90% identical to SEQ ID NO:
- 3.
An isolated and purified nucleic acid sequence that is at least 90% identical to a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 2 4, 5, 6, 7, 8, 9, and 10.
A protein encoded by a nucleic acid selected from the group consisting of SEQ ID NOs:
- 2 4, 5, 6, 7, 8, 9, and 10 and variants thereof that are at least 80% identical to SEQ ID NOs:
- 2 4, 5, 6, 7, 8, 9, and 10, wherein said protein has at least one activity of RICK3.
A method for producing variants of RICK3 comprising:
- a) providing a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 24,5,6,7,8,9,and 10;
- b) mutating said nucleic acid sequence to generate a variant of RICK3;
- and
- c) screening said variant for RICK3 activity.
A composition comprising a nucleic acid that inhibits the binding of at least a portion of a nucleic acid selected from the group consisting of SEQ ID NOs:
- 2 4, 5, 6, 7, 8, 9, and 10 to their complementary sequences.
A method for screening compounds, comprising:
- a) providing:
- i) a first polypeptide sequence comprising at least a portion of PKK;
- ii) a second polypeptide sequence comprising at least a portion of a protein known to interact with PKK;
- and
- iii) one or more test compounds;
- b) combining in any order, said first polypeptide sequence comprising at least a portion of PKK, said second polypeptide sequence comprising at least a portion of a protein known to interact with PKK, and said one or more test compounds under conditions such that said first polypeptide sequence, said second polypeptide sequence, and said test compound interact;
- and
- c) detecting the presence or absence of an interaction between said polypeptide sequence comprising at least a portion of PKK and said polypeptide sequence comprising at least a portion of a protein known to interact with PKK.
A method for screening compounds, comprising:
- a) providing:
- i) a polypeptide sequence comprising at least a portion of a polypeptide selected from the group consisting of PKK and RICK3, wherein said polypeptide comprises protein kinase activity;
- and
- iii) one or more test compounds;
- and
- b) contacting said test compound and said polypeptide;
- and
- c) detecting the level of kinase activity of said polypeptide.
Dependencies are inferred from the claim text.