ICAM-related protein
OwnerW. Michael Gallatin, Rosemay Vazeuxinventors
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Abstract
DNA sequences encoding a novel human intercellular adhesion molecule polypeptide (designated “ICAM-R”) and variants thereof are disclosed along with methods and materials for production of the same by recombinant procedures. Binding molecules specific for ICAM-R and variants thereof are also disclosed as useful in both the isolation of ICAM-R from natural cellular sources and the modulation of ligand/receptor binding biological activities of ICAM-R.
Claims5
Hybridoma cell line ATCC HB 12190.
A method for identifying a compound that modulates the interaction of binding partners ICAM-R and CD11b, said method comprising the steps of:
- (a) immobilizing ICAM-R or CD11b;
- (b) detectably labeling the non-immobilized binding partner;
- (c) contacting said immobilized binding partner with said labelled binding partner in the presence and absence of a test compound;
- (d) detecting label bound to said immobilized binding partner;
- (d) identifying a modulating compound as a test compound that affects the label bound in the presence of said test compound in comparison to the label bound in the absence of said test compound.
A method for identifying a compound that modulates the interaction of binding partners ICAM-R and CD11b comprising the steps of:
- (a) immobilizing ICAM-R or CD11b on a solid support coated or impregnated with a fluorescent agent;
- (b) labelling the non-immobilized binding partner with a chemical capable of exciting said fluorescent agent;
- (c) contacting said immobilized binding partner with said labelled binding partner in the presence and absence of a test compound;
- (d) detecting light emission by said fluorescent agent;
- and
- (e) identifying a modulating compound as a test compound that affects the label bound in the presence of said test compound in comparison to the label bound in the absence of said test compound.
A method for identifying a compound that modulates phosphorylation of ICAM-R by protein kinase C isoform comprising the steps of:
- (a) exposing ICAM-R peptide comprising amino acids 482 to 518 of SEQ ID NO:
- 1 to protein kinase C isoform and labeled phosphate in the presence and absence of a test compound;
- (b) measuring labeled phosphate transferred to said ICAM-R peptide;
- and
- (c) identifying a test compound that affects transfer of said labeled phosphate as an modulator compound.
Dependencies are inferred from the claim text.