Methods for increasing mRNA half-life in eukaryotic cells
AssigneeThe Regents of the University of California
InventorDavid L. Meyer
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Abstract
A method is provided whereby altered cells exhibit increased intracellular half-life of transcribed mRNAs resulting in increased levels of expressed and/or secreted proteins. The cells are genetically altered to increase the level of intracellular ribosome receptor, which induces mRNA half-life.
Claims52
A method of increasing the intracellular half-life of an mRNA in a eukaryotic cell, said method comprising
- increasing the intracellular level of a ribosome receptor, as compared to the level of said ribosome receptor in an unmodified or untreated eukaryotic cell, whereby the increase in intracellular level of said ribosome receptor results in an increase in the half-life of said mRNA.
A method of increasing production of a protein by a eukaryotic cell, said method comprising:
- increasing the intracellular half-life of an mRNA encoding said protein by increasing the intracellular level of a ribosome receptor in said cell, as compared to the level of said ribosome receptor in an unmodified or untreated eukaryotic cell of the same type, whereby the increase in intracellular level of said ribosome receptor results in an increase in the half-life of said mRNA and resulting in an increase in expression of said protein.
A kit for increasing the half-life of an mRNA in a eukaryotic cell, said kit comprising:
- a container containing a nucleic acid construct encoding one or more ribosome receptors;
- and
- instructional materials teaching that increasing the intracellular level of a ribosome receptor will increase the intracellular half-life of an mRNA.
A kit for increasing the half-life of an mRNA in a eukaryotic cell, said kit comprising:
- a container containing a eukaryotic cell that has been treated or modified to express an elevated level of ribosome receptor as compared to an untreated or unmodified cell of the same type;
- and
- instructional materials teaching that increasing the intracellular level of a ribosome receptor will increase the intracellular half-life of an mRNA.
Dependencies are inferred from the claim text.