Subtilase variants having an improved wash performance on egg stains

Use according to any of the preceding claims, wherein the variant comprises at least one further modification.

Use according to any of the preceding claims, wherein the parent subtilase belongs to the sub-group I-S1.

A subtilase variant selected from the group consisting of

• a variant comprising at least one additional amino acid residue in the active site (b) loop corresponding to the insertion of at least one additional amino acid residue between positions 98 and 99 and further comprising at least one additional modification (BASBPN numbering), and
• a variant comprising at least one additional amino acid residue in the active site (b) loop corresponding to the insertion of at least one additional amino acid residue between positions 99 and 100 and further comprising at least one additional modification (BASBPN numbering),
• where the variant—when tested in the “Ovo-inhibition Assay” disclosed in Example 4 herein—has a Residual Activity of at least 10%.

A method for removal of egg stains from a hard surface or from laundry, the method comprising contacting the egg stain-containing hard surface or the egg stain-containing laundry with a cleaning or detergent composition, preferably a laundry or dishwash composition, containing a subtilase variant comprising at least one additional amino acid residue in the active site loop (b) region from position 95 to 103 (BASBPN numbering).

Use of a cleaning or detergent composition, preferably a laundry or dishwash composition, containing a subtilase variant comprising at least one additional amino acid residue in the active site loop (b) region from position 95 to 103 (BASBPN numbering) for removal of egg stains from laundry or from hard surfaces.