Crystallization of ribosome recycling factor (RRF) and method of developing inhibitor of RRF

Abstract

There are disclosed a crystal of complex of RRF with a detergent, its crystallization method and a method for identifying an inhibitor for RRF by using the three-dimensional structure of RRF analyzed from the crystal. In the present invention, a crystal of RRF complexed with a detergent is obtained in the presence of the detergent, and the three-dimensional structure of RRF is analyzed from the crystal. There are identified an active site forming hydrophobic cleft and binding site of RRF to A-site of ribosome in the three-dimensional structure of RRF. The three-dimensional structure of RRF, specifically, the active site and binding site can allow an inhibitor for RRF to be designed and identified efficiently. RRF is essential for viability in prokaryotes, but is not essential in eukaryotes. From this fact, the inhibitor for RRF can be used as an antibiotic.

Dependencies are inferred from the claim text.