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AssigneeCorning Incorporated
InventorsDana Craig Bookbinder, Edward John Fewkes JR., James Arthur Griffin, Frances M. Smith, David L. Tennent
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Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.
A method for treating a hydrophobic surface so as to reduce the ability of an organic material in an aqueous solution to bind to the surface, said method comprising:
An article of manufacture comprising a hydrophobic surface upon which a non-ionic surfactant has been dried, said non-ionic surfactant having (i) a hydrophilic-lipophilic balance number which is less than or equal to 5 and (ii) at least one hydrophilic element which can extend into an aqueous solution.
A method for making a body composed of a hydrophobic polymer, said method comprising:
A method for reducing the loss of biological activity of an organic molecule in an aqueous solution due to contact with hydrophobic surfaces comprising:
A method for reducing the ability of an organic material in an aqueous solution to bind to a hydrophobic surface comprising:
A product made from a polymer blend comprising:
A method of manufacturing a polymer blend capable of reducing the ability of an organic material in an aqueous solution to bind to its surface, comprising the steps of:
An article of manufacture comprising a surface upon which a non-ionic surfactant has bloomed, said non-ionic surfactant having (i) a hydrophilic-lipophilic balance number which is less than or equal to 10 and (ii) at least one hydrophilic element which can extend into an aqueous solution.
Dependencies are inferred from the claim text.