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US 2003/0199048 A1Published application
Stereoselective estarase from aspergillus oryzae
AssigneeNovozymes A/S
InventorsPeter Rahbek Ostergaard, Carsten M Hjort, Heinz-Josef Deussen, Magali Zundel, Soren Ebdrup, Soren Christensen, Shamkant Anant Patkar
Life of this patent
Estimating the term…
Abstract
An esterase isolated from Aspergillus oryzae is capable of stereoselective hydrolysis of chiral esters and also has arylesterase activity (EC 3.1.1.2) and feruloyl esterase activity (EC 3.1.1.73). The esterase has only a limited homology to known amino acid sequences.
Claims13
An esterase which is:
- a) a polypeptide encoded by an esterase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli deposit number DSM 13977;
- b) a polypeptide having an amino acid sequence as the mature peptide shown in seq id no:
- 2, or which can be derived therefrom by substitution, deletion, and/or insertion of one or more amino acids;
- c) an analogue of the polypeptide defined in (a) or (b) which:
- i) has at least 50% identity with said polypeptide,
- ii) is immunologically reactive with an antibody raised against said polypeptide in purified form, or
- iii) is an allelic variant of said polypeptide;
- or
- d) a polypeptide which is encoded by a nucleic acid sequence which 15 hybridizes at 60° C., 2× SSC, 0.5% SDS with a complementary strand of the nucleic acid sequence shown as nucleotides 629-911 and 971-2208 of SEQ ID NO:
- 1 or a subsequence thereof having at least 100 nucleotides.
7 more elements
A polynucleotide which comprises:
- a) the esterase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli DSM 13977,
- b) the nucleic acid sequence shown as nucleotides 629-911 and 971-2208 of SEQ ID NO:
- 1,
- c) an analogue of the sequence defined in a) or b) which encodes a esterase and
- i) has at least 60% identity with said DNA sequence, or
- ii) hybridizes at 60° C., 2× SSC, 0.5% SDS with a complementary strand of said DNA sequence or a subsequence thereof having at least 100 nucleotides,
- iii) is an allelic variant thereof, or
- d) a complementary strand of a), b) or c).
5 more elements
The method of the preceding claim wherein the esterase can be derived from the mature peptide of SEQ ID NO:
- 2 or is an analogue thereof, and the host cell is a transformed strain of A. oryzae.
Dependencies are inferred from the claim text.