Strains of saccharaothrix, process for producing pravastain using the strains and isolation process of (HMG)-CoA reductase
AssigneeYung Shin Pharmaceutical Ind. Co., Ltd.
InventorsFang-Yu Lee, Ming-Liang Lee, Anderson C. Hong, Schung-Ching Chiu
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Abstract
The present invention provides two new microorganism strains of Saccharothrix, designated as YS-44442 and YS-45494, a process of producing pravastatin using the strains, and an improved process for isolation of (HMG)-CoA reductase inhibitors.
Claims25
A microorganism strain YS-44442 of Saccharothrix and the mutant thereof.
A microorganism strain YS-45494 of Saccharothrix and the mutant thereof.
A process of isolating pravastatin, comprising the steps of (1) adding an ammonium sulfate into a first solution containing the(HMG)-CoA reductase inhibitor to produce a precipitation;
- (2) isolating the precipitation;
- (3) dissolving the precipitation with a polar solvent to produce a second solution;
- (4) adjusting the pH of the second solution to about pH 4 to about PH 6;
- and (5) extracting the second solution with an water immiscible solvent to isolate the (HMG)-CoA reductase inhibitor.
Dependencies are inferred from the claim text.