Process for the preparation of L-amino acids with amplification of the zwf gene
OwnerKevin Burke, Hermann Sahm +8inventors
InventorsKevin Burke, Hermann Sahm, Lothar Eggeling, Bernd Moritz, L. K. Dunican, Ashling McCormack, Cliona Stapelton, Bettina Mockel, Georg Thierbach, Rita Dunican
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Abstract
The invention relates to a process for the preparation of L-amino acids. The process involves fermenting an L-amino acid producing coryneform bacteria in a culture medium, concentrating L-amino acid in the culture medium or in the cells of the bacteria, and isolating the L-amino acid produced. The bacteria has an amplified gene encoding the Zwischenferment protein.
Claims16
A process for the preparation of L-lysine comprising:
- a) fermenting an L-lysine producing coryneform bacteria in a culture medium, the bacteria having at least an overexpressed zwf gene encoding the Zwischenferment protein;
- b) concentrating the L-lysine in the culture medium or in the cells of the bacteria;
- and
- c) isolating the L-lysine produced;
- wherein intracellular activity of pyruvate oxidase encoded by the poxB gene is decreased or switched off in the bacteria.
A process for the preparation of L-amino acids comprising:
- a) fermenting an L-amino acid producing bacteria in a culture medium, the bacteria having at least an overexpressed zwf gene encoding the Zwischenferment protein;
- b) concentrating the L-amino acid in the culture medium or in the cells of the bacteria, and
- c) isolating the L-amino acid produced;
- wherein the intracellular activity of the pyruvate oxidase encoded by the poxB gene is decreased or switched off in the bacteria;
- and
- wherein the L-amino acid is selected from the group consisting of L-threonine, L-isoleucine and L-tryptophane.
A process for the preparation of L-lysine, comprising:
- a) fermenting an L-lysine producing bacteria in a culture medium, the bacteria having at least an overexpressed zwf gene encoding the Zwischenferment protein;
- b) concentrating the L-lysine in the culture medium or in the cells of the bacteria;
- and
- c) isolating the L-lysine produced;
- wherein intracellular activity of the glucose 6-phosphate isomerase encoded by the pgi gene is decreased or switched off in the bacteria.
A process for the preparation of L-amino acids comprising:
- a) fermenting an L-amino acid producing bacteria in a culture medium, the bacteria having at least an overexpressed zwf gene encoding the Zwischenferment protein;
- b) concentrating the L-amino acid in the culture medium or in the cells of the bacteria;
- and
- c) isolating the L-amino acid produced;
- wherein intracellular activity of the glucose 6-phosphate isomerase encoded by the pgi gene is decreased or switched off in the bacteria;
- and
- wherein the L-amino acid is selected from the group consisting of L-threonine, L-isoleucine and L-tryptophane.
An L-amino acid producing coryneform microorganism having increased intracellular activity of the Zwischenferment protein and decreased intracellular activity of pyruvate oxidase.
An L-amino acid producing coryneform microorganism having increased intracellular activity of the Zwischenferment protein and decreased intracellular activity of glucose 6-phosphate isomerase.
The DNA of SEQ ID NO:
- 9 containing nucleotides 538 to 2079.
The plasmid vector pEC-T18mob2 deposited under the designation DSM13244 in E. coli K-12 DH5α.
Dependencies are inferred from the claim text.