Single chain trimers of class I MHC molecules
OwnerTed H. Hanseninventor
Life of this patent
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Abstract
A recombinant DNA molecule is comprised of a DNA sequence that encodes a single chain trimer of a novel mature class I MHC molecule. The single chain trimer contains, in sequence from the N-terminus to the C-terminus: a peptide ligand segment; (2) a first linker; (3) a β2m segment; (4) a second linker; and (5) a class I heavy chain segment, wherein the peptide ligand segment has a carboxy end, the β2m segment has amino and carboxy ends, and the heavy chain segment has an amino end, and wherein the peptide ligand segment is covalently linked via its carboxy end to the amino end of the β2m segment by the first linker, and wherein the β2m segment is covalently linked via its carboxy end to the amino end of the heavy chain segment by the second linker.
Claims45
A recombinant DNA molecule comprising a DNA sequence encoding a single chain trimer of a mature MHC molecule, the single chain trimer comprising in sequence:
- (1) a peptide ligand segment;
- (2) a first linker;
- (3) a β2m segment;
- (4) a second linker;
- and
- (5) a class I heavy chain segment,
- wherein the peptide ligand segment has a carboxy end, the β2m segment has amino and carboxy ends, and the heavy chain segment has an amino end, and wherein the peptide ligand segment is covalently linked via its carboxy end to the amino end of the β2m segment by the first linker, and wherein the β2m segment is covalently linked via its carboxy end to the amino end of the heavy chain segment by the second linker.
A recombinant DNA molecule comprising a DNA sequence encoding a single chain trimer of a mature MHC molecule, the single chain trimer comprising in sequence:
- (1) an antigenic peptide ligand segment containing from about 4 to 30 amino acid residues;
- (2) a first linker comprising at least 10 amino acid residues;
- (3) a β2m segment;
- (4) a second linker comprising at least 15 amino acid residues;
- and
- (5) a heavy chain segment comprising an HLA-A, HLA-B, HLA-C, 1a, 1b, H-2-K, H-2-Dd or H-2-Ld heavy chain,
- wherein the peptide ligand segment has a carboxy end, the β2m segment has amino and carboxy ends, and the heavy chain segment has an amino end, and wherein the peptide ligand segment is covalently linked via its carboxy end to the amino end of the β2m segment by the first linker, and wherein the β2m segment is covalently linked via its carboxy end to the amino end of the heavy chain segment by the second linker.
A class I heavy chain containing a mutated conserved residue.
A single chain trimer of a mature Class I MHC molecule comprising
- (1) a peptide ligand segment having a carboxy end;
- (2) a first linker;
- (3) a β2M segment having amino and carboxy ends;
- (4) a second linker;
- and
- (5) a class I heavy chain segment having an amino end, wherein the β2m segment and the heavy chain segment are encoded by a mammalian Class I MHC locus, wherein the carboxy end of the peptide ligand segment is covalently linked to the amino end of the β2m segment via a first flexible peptide linker, and wherein the carboxy end of the β2m segment is covalently linked to the amino end of the class I heavy chain segment via a second flexible peptide linker.
A single chain trimer of a mature Class I MHC molecule comprising:
- (1) an antigenic peptide ligand segment containing from about 4 to 30 amino acid residues and having a carboxy end
- (2) a first linker comprising at least 10 amino acid residues;
- (3) a β2m segment having amino and carboxy ends;
- (4) a second linker comprising at least 10 amino acid residues;
- and
- (5) a heavy chain segment comprising an HLA-A, HLA-B, HLA-C, 1a, 1b, H-2-K, H-2-Dd, and H-2-Ld heavy chain having an amino end, wherein the β2m segment and the heavy chain segment are encoded by a mammalian Class I MHC locus, wherein the carboxy end of the peptide ligand segment is covalently linked to the amino end of the β2m segment via a first flexible peptide linker, and wherein the carboxy end of the β2m segment is covalently linked to the amino end of the class I heavy chain segment via a second flexible peptide linker.
A mutein of a class I heavy chain molecule having an amino acid other than tyrosine substituted at position 84.
Dependencies are inferred from the claim text.