Sample preparation of biological fluids for proteomic applications
OwnerAldo M. Pitt, Sara D. Gutierrezinventors
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Abstract
A system, kit and process for the separation, recovery, purification and identification of biomarkers such as peptides and the like from serum or plasma. The process is to filter the sample through a UF filtration device optionally having a vertical or substantial vertical ultrafilter membrane, especially when in a single well configuration device. The filtrate is recovered and if desired desalted using various chromatography media such as reverse phase and ion exchange media. The sample is then applied to a MALDI/TOF target or HPLC column and analyzed in a mass spectrometer the presence, absence or variation of the biomarkers. Higher recovery and concentration of these biomarkers is achieved with the present invention.
Claims26
1) A process for the sample preparation of biological fluids for proteomic applications comprising the steps of selecting a biological fluid, placing the fluid in an ultrafiltration membrane containing filtration device wherein the filter is an ultrafiltration membrane having a nominal molecular weight cutoff equal to or less than about 100 kiloDaltons (kD), applying a filtration force to the fluid in the device to create a filtrate that is essentially free of high molecular weight constituents and recovering the filtrate, and applying mass spectrometry analysis to the recovered filtrate.
2) A process for the sample preparation of biological fluids for proteomic applications comprising the steps of selecting a biological fluid, placing the fluid in an ultrafiltration membrane containing filtration device wherein the major plane of the filter to the direction of force applied to the filter is from about −60 to +60 degrees from the direction of the force vector and the filter is an ultrafiltration membrane having a nominal molecular weight cutoff equal to or less than about 100 kiloDaltons(kD), applying a filtration force to the fluid in the device to create a filtrate that is essentially free of high molecular weight constituents and recovering the filtrate, concentrating and desalting the filtrate and applying mass spectrometry analysis to the recovered filtrate.
15) A kit for the isolation and purification of biomarkers comprising a filtration well device, wherein the filtration device has one or more wells, each well has one or more ultrafiltration membranes having a nominal molecular weight cutoff equal to or less than about 100 kiloDaltons (kD), one or more filtrate collection wells downstream of the one or more wells, and one or more portions of chromatography media for the desalting of the filtrate.
Dependencies are inferred from the claim text.