Forward screening with minicells
OwnerRoger A. Sabbadini, Neil Berkley +2inventors
InventorsRoger A. Sabbadini, Neil Berkley, Mark W. Surber, William Gerhart
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Abstract
The invention provides compositions and methods for the production of achromosomal and anucleate cells useful for applications such as diagnostic and therapeutic uses, as well as research tools and agents for drug discovery.
Claims15
A method of identifying an agent that effects the activity of a protein, comprising contacting a library of two or more candidate agents with a minicell comprising said protein or a polypeptide derived from said protein, assaying the effect of candidate agents on the activity of said protein, and identifying agents that effect the activity of said protein.
A method of identifying an agent that effects the activity of a protein domain containing a library of two or more candidate agents with a minicell displaying a membrane fusion protein, said fusion protein comprising a first polypeptide, said first polypeptide comprising at least one transmembrane domain or at least one membrane anchoring domain, and a second polypeptide, wherein said second polypeptide comprises said protein domain.
A method of identifying undesirable side-effects of a biologically active compound that occur as a result of binding of said compound to a protein, wherein binding a compound to said protein is known to result in undesirable side effects, comprising contacting a minicell that comprises said protein to said biologically active compound.
Dependencies are inferred from the claim text.