Androgen receptor coactivators
AssigneeUniversity of Rochester
InventorChawnshang Chang
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Abstract
Disclosed are androgen receptor-associated proteins, designated ARA24, ARA54, ARA55, and Rb, that have been demonstrated to interact with the androgen receptor to alter levels of androgen receptor-mediated transcriptional activation. Certain of these proteins interact with the androgen receptor in an androgen-dependent manner, whereas certain proteins may induce transcriptional activation in the presence of other ligands, such as E2 or HF. Also disclosed is a method of detecting androgenic or antiandrogenic activity using these proteins in a mammalian two-hybrid transient transfection assay.
Claims12
An isolated polynucleotide comprising a sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:3.
A genetic construct comprising a promoter capable of causing expression of a protein coding region in a cell, the promoter operably connected to a protein coding region encoding the expression of a polypeptide from coding regions of ARA54 or ARA55.
A method for testing the androgenic or antiandrogenic effect of a chemical compound comprising the steps of:
- (a) transfecting a host cell with at least one genetic construct capable of producing in the host cell a polypeptide selected from the group consisting of ARA54, ARA55, ARA24, and Rb, the host cell also producing human androgen receptor protein;
- (b) exposing the cell to the chemical compound;
- and
- (c) measuring the level of transcriptional activity caused by the androgen receptor.
A method for testing the androgenic or antiandrogenic effect of a chemical compound comprising the steps of:
- (a) transfecting a host cell with at least one genetic construct capable of producing in the host cell human androgen receptor protein and a polypeptide selected from the group consisting of ARA54, ARA55, ARA24, and Rb;
- (b) exposing the cell to the chemical compound;
- and
- (c) measuring the interaction between AR and an AR co-activator.
Dependencies are inferred from the claim text.