Methods for designing oligo-probes with high hybridization efficiency and high antisense activity
OwnerOlga V. Matveevainventor
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Abstract
Methods are disclosed for selecting oligonucleotide probes with high target affinity. These methods include combining several types of thermodynamic criteria or nucleotide composition criteria for the selection of efficient oligonucleotide probes. The development of these methods was based on statistical analysis of datasets of hybridization or antisense experiments. The methods described here demonstrated variable efficiency in practice, however any or all of them can be used depending on the task of probe design.
Claims19
A method for designing oligo-probes with a high target affinity, said method comprising the steps of:
- a. learning;
- b. testing;
- and
- c. filtering.
A method for designing oligo-probes capable of interacting with a high antisense efficiency, said method comprising the steps of:
- a. learning;
- b. testing;
- and
- c. filtering.
A method for designing oligo-probes with high target affinity for hybridization experiments or antisense efficiency comprising the steps of:
- a. calculating thermodynamic parameters for each of a plurality of oligo-probe candidates;
- b. filtering said oligo-probe candidates to provide a subset of oligo-probes;
- and
- c. designing a group of oligo-probes that includes said subset of oligo-probes.
Dependencies are inferred from the claim text.