Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof
AssigneeBTG International Limited
InventorsRichard Lathe, Kenneth Andrew Rose, Genevieve Stapleton
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Abstract
This invention provides novel hippocampus-associated proteins and DNA sequences coding therefor. In an investigation of hippocampus-associated proteins by differential screening of a rat hippocampus cDNA library, a cDNA species encoding a novel protein designated Hct-1 was isolated and shown to be a to cytochromes P450. The use of hybridization probes based on the rat Hct-1 sequence has led to the identification of homologues in other mammalian species.
Claims25
A DNA molecule selected from the following:
- (a) DNA molecules containing the coding sequence set forth in SEQ Id No:
- 1 beginning at nucleotide 22 and ending at nucleotide 1541,
- (b) DNA molecules containing the coding sequence set forth in SEQ Id No:
- 2 beginning at nucleotide 1 and ending at nucleotide 1242,
- (c) DNA molecules capable of hybridizing with the DNA molecule defined in (a) or (b) under standard hybridization conditions defined as 2×SSC at 65° C.
- (d) cytochrome P450-encoding DNA molecules capable of hybridizing with the DNA molecule defined in (a), (b) or (c) under reduced stringency hybridization conditions defined as 6×SSC at 55° C.
A DNA molecule comprising a human Hct-1 gene-associated sequence and selected from the following:
- (e) DNA molecules comprising one or more sequences selected from
- (i) the sequence designated “intron 2” in SEQ Id No 3,
- (ii) the sequence designated “exon 3” in SEQ Id No 3,
- (iii) the sequence designated “intron 3” in SEQ Id No 3,
- (iv) the sequence designated “exon 4” in SEQ Id No 3, and
- (v) the sequence designated “intron 5” in SEQ Id No 3;
- and
- (f) DNA molecules capable of hybridizing with the DNA molecules defined in (e) under standard hybridization conditions defined as 2×SSC at 65° C.
- (g) cytochrome P450-encoding DNA molecules capable of hybridizing with the DNA molecule defined in (e) or (f) under reduced stringency hybridization conditions defined as 6×SSC at 55° C.
A DNA molecule comprising a human Hct-1 gene-associated sequence and selected from the following:
- (h) DNA molecules comprising contiguous pairs of sequences selected from
- (i) the sequence designated “intron 2” in SEQ Id No 3,
- (ii) the sequence designated “exon 3” in SEQ Id No 3,
- (iii) the sequence designated “intron 3” in SEQ Id No 3,
- (iv) the sequence designated “exon 4” in SEQ Id No 3, and
- (v) the sequence designated “intron 5” in SEQ Id No 3;
- and
- (i) DNA molecules capable of hybridizing with the DNA molecules defined in (h) under standard hybridization conditions defined as 2×SSC at 65° C.
- (j) cytochrome P450-encoding DNA molecules capable of hybridizing with the DNA molecule defined in (h) or (i) under reduced stringency hybridization conditions defined as 6×SSC at 55° C.
A DNA molecule comprising a human Hct-1 gene-associated coding sequence selected from the following:
- (k) DNA molecules comprising a contiguous coding sequence consisting of the sequences “exon 3” and “exon 4” in SEQ Id No 3, and
- (l) DNA molecules capable of hybridizing with the DNA molecules defined in (k) under standard hybridization conditions defined as 2×SSC at 65° C.
- (m) cytochrome P450-encoding DNA molecules capable of hybridizing with the DNA molecule defined in (k) or (l) under reduced stringency hybridization conditions defined as 6×SSC at 55° C.
A DNA molecule consisting of a contiguous sequence of at least 1-8 nucleotides from the DNA sequence set forth in SEQ Id Nos:
- 1, 2 and 3.
A protein selected from the following:
- (i) the protein designated rat Hct-1 comprising the amino acid sequence set forth in SEQ Id No:
- 1 or a protein having substantial homology thereto,
- (ii) the protein designated mouse Hct-1 comprising the amino acid sequence set forth in SEQ Id No:
- 2 or a protein having substantial homology thereto, or
- (iii) the protein designated human Hct-1 comprising the amino acid sequence set forth in SEQ Id No:
- 3:
- or a protein having substantial homology thereto.
An antibody, especially a monoclonal antibody which binds to a Hct-1-protein.
Dependencies are inferred from the claim text.