Assay for nitrous oxide neurologic syndrome
OwnerKirk J. Hogan, Rebecca M.R. Selzerinventors
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Abstract
A method for detection of susceptibility to nitrous oxide neurologic syndrome in a subject is disclosed. In one embodiment, the method comprises: (a) providing a sample from a subject, wherein said subject is a candidate for nitrous oxide anesthesia; and (b) detecting the presence or absence of folate, cobalamin, methionine and homocysteine pathway genetic polymorphisms in said sample, wherein the presence of a polymorphism indicates that the subject is susceptible to nitrous oxide neurologic syndrome.
Claims31
A method for detection of susceptibility to nitrous oxide neurologic syndrome in a subject, comprising:
- a) providing a sample from a subject, wherein said subject is a candidate for nitrous oxide exposure;
- and
- b) detecting the presence or absence of folate, cobalamin, methionine and homocysteine pathway genetic polymorphisms in said sample, wherein the presence of a polymorphism indicates that the subject is susceptible to nitrous oxide neurologic syndrome.
A kit comprising a reagent for detecting the presence or absence of folate, cobalamin, methionine and homocysteine pathway genetic polymorphisms in a sample, wherein the reagent is a nucleic acid molecule comprising at least 11 nucleotides of the MTHFR, MTR, MTRR or CBS genes or their complement.
A method of diagnosing a mutation in the human 5,10-methylene tetrahydrofolate reductase gene comprising the step of examining a patient's 5,10-methylene tetrahydrofolate reductase gene and determining whether a polymorphism exists in residue 1755.
A method of diagnosing 5,10-methylene tetrahydrofolate reductase deficiency in a human patient comprising examining a patient's 5,10-methylene tetrahydrofolate reductase gene and determining whether a polymorphism exists.
A gene probe useful to detect a mutation in the 5,10-methylene tetrahydrofolate reductase gene, comprising at least 11 nucleotides of SEQ ID NO:1 or the complement of this sequence, wherein the sequence includes residue 1755.
Dependencies are inferred from the claim text.