Heteroconfigurational polynucleotides and methods of use

The composition of any one of the preceding claims, wherein the L-form polynucleotide sequence portion comprises at least one L-form 2′-4′ LNA nucleotide.

The composition of any one of the preceding claims, wherein the D-form polynucleotide sequence portion comprises at least one D-form 2′-4′ LNA nucleotide.

The composition of any one of the preceding claims, wherein the D-form polynucleotide sequence portion comprises at least one L-form nucleotide comprising ribose, 2′-deoxyribose, 2′,3′-dideoxyribose, 2′-fluororibose, 2′-chlororibose, or 2′-O-methylribose.

The composition of any one of the preceding claims, wherein at least one of the D-form polynucleotide sequence portion and the L-form polynucleotide sequence portion comprises an internucleotide linkage selected from a 2-aminoethylglycine, a phosphorothioate, a phosphorodithioate, a phosphotriester, and a phosphoramidate.

The composition of any one of the preceding claims, wherein the heteroconfigurational polynucleotide comprises a nucleobase selected from uracil, thymine, cytosine, adenine, 7-deazaadenine, guanine, and 7-deazaguanosine.

The composition of claim any one of the preceding claims, which comprises a first complementary polynucleotide that is hybridized to the L-form polynucleotide sequence portion.

The composition of any one of the preceding claims, which comprises a second complementary polynucleotide that is hybridized to the D-form polynucleotide sequence portion.

The composition of any one of the preceding claims, which comprises a detectable label.

An array of different-sequence polynucleotides comprising 5 to 100 L-nucleotides, wherein the polynucleotides are immobilized at addressable locations on a solid support.

A method of forming a polynucleotide hybrid comprising

• providing a heteroconfigurational polynucleotide comprising a D-form polynucleotide sequence portion and an L-form polynucleotide sequence portion that is covalently linked to the D-form polynucleotide sequence portion, and hybridizing the heteroconfigurational polynucleotide to a first complementary polynucleotide to form a duplex between the first complementary polynucleotide and the L-form polynucleotide sequence portion.

The method of any one of claims 57 to 72, wherein the first complementary polynucleotide comprises at least one L-form nucleotide.