Multi-functional microarrays and methods
OwnerRobert S. Matson, Raymond C. Milton +2inventors
InventorsRobert S. Matson, Raymond C. Milton, Robert J. Obremski, John W. Silzel
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Abstract
Detection devices for multianalyte detection on a solid substrate, methods for the preparation of the devices and their use in analytical and diagnostic procedures are described. The detection devices include a solid substrate fabricated with an array of detection spots, the detection spots having an analyte sensor bound to the substrate by a universal binding ligand. The universal binding ligand is capable of binding multiple analyte sensors to create a multifunctional array. A process for producing the detection devices and assay methods employing microprinting technology are also described.
Claims26
An analyte detection device comprising:
- a) a substrate;
- and
- b) an array of detection spots on the substrate, each detection spot comprising an analyte sensor and a first binding ligand;
- wherein
- i. the analyte sensor is bound to the substrate by the binding ligand;
- ii. there are a plurality of analyte sensors for different analytes;
- and
- iii. the same first binding ligand is used for two, or more than two different analyte sensors.
A method for detecting a plurality of different analytes in a sample comprising:
- a) selecting an analyte detection device comprising:
- i. a substrate;
- and
- ii. an array of detection spots on the substrate, wherein each detection spot comprises an analyte sensor and a binding ligand, and wherein the analyte sensor is bound to the substrate by the binding ligand;
- and
- b) printing more than one analytical sample substantially only on each detection spot.
A method for preparing an analyte detection device comprising placing a plurality of analyte sensors on an array of binding ligand spots, wherein each analyte sensors is for a different analyte, and wherein the same binding ligand is used for two, or more than two different analyte sensors, and wherein each analyte sensor is placed substantially only on each binding ligand spot by printing.
A method for detecting analytes in a sample comprising placing an analytical sample on an array of analyte sensor spots, wherein the array of analyte sensor spots is comprised of two or more than two different analyte sensor spots, and the analytical sample is placed substantially only on each analyte sensor spot by printing.
A method for multi-step synthesis comprising:
- a) selecting a substrate;
- b) placing an array of binding ligand spots on the substrate;
- and
- c) placing one or more than one synthetic reagents on the array of binding ligand spots, wherein there are a plurality of different reagents for different syntheses, and wherein the same binding ligand is used for two or more than two different syntheses, and wherein the synthetic reagents are placed substantially only on each binding ligand spot by printing.
Dependencies are inferred from the claim text.