Displacement assay for detection of small molecules
OwnerMilan N. Stojanovic, Donald W. Landryinventors
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Abstract
Complex of an anti-cocaine aptamer and the dye diethylthiotricarbocyanine behaves as a colorimetric sensor with attenuation in absorbance at 760 nm for cocaine in the concentration range of 2-5000 μM. Mechanistic studies indicate an intermolecular displacement of the dye as the mechanism of action of the sensor. As the dye is insoluble in buffer, cocaine binding can be detected as displaced dye precipitates and supernatant decolorizes.
Claims69
A sensor for detecting the presence of a compound, which comprises an oligonucleotide receptor capable of binding to the compound, wherein the receptor comprises a dye attached to the receptor which is released when the receptor binds to the compound.
A method of detecting the presence of a compound in solution which comprises:
- (a) providing an oligonucleotide receptor with an attached dye
- (b) contacting the oligonucleotide receptor with the compound,
- (c) releasing the dye from the oligonucleotide receptor, and
- (d) detecting the presence of the released dye,
- wherein the presence of the released dye indicates the presence of the compound in the solution.
A method of determining the concentration of a compound in solution which comprises:
- (a) providing an oligonucleotide receptor with an attached dye
- (b) contacting the oligonucleotide receptor with the compound,
- (c) releasing the dye from the oligonucleotide receptor, and
- (d) measuring a change in absorbance of the dye,
- wherein the change in absorbance indicates the concentration of the compound in solution.
Dependencies are inferred from the claim text.