Probe bound substrate, process for manufacturing same, probe array, method of detecting target substance, method of specifying nucleotide sequence of single-stranded nucleic acid in sample, and quantitative determination of target substance in sample
OwnerTadashi Okamoto, Nobuko Yamamoto +1inventors
InventorsTadashi Okamoto, Nobuko Yamamoto, Tomohiro Suzuki
Life of this patent
Estimating the term…
Abstract
A probe bound substrate allowing us to quickly detect or quantify a target substance or sequence a target nucleic acid at a lower cost is provided. Specifically, there is provided a probe bound substrate in which a probe capable of specifically attaching to a target substance is bound at the first site on its surface, characterized in that a marker is bound at the second site where the first site may be specified.
Claims59
A probe bound substrate on which a probe capable of specifically attaching to a target substance is bound at a first site on a surface of the substrate, characterized in that a marker is bound at a second site where the first site can be specified.
A process for manufacturing a probe bound substrate comprising the steps of:
- applying a solution containing a probe capable of specifically making a bond with a target substance and having a second functional group capable of making a bond with a first functional group attached to the surface of a substrate, to a first site of a surface of a substrate and binding the probe to the substrate at the first site of a substrate surface, further comprising the step of:
- applying a solution containing a marker having a third functional group capable of directly or indirectly making a bond with said first functional group to a second site of the substrate surface binding said maker to the second position of said substrate surface and wherein said first site can be specified from said second site.
A probe array comprising spots for mutually independent probes at multiple sites on a substrate surface wherein a marker is present on the substrate surface such that the positions of said spots can be specified.
A method of detecting a target substance comprising the steps of:
- contacting a sample with each spot in a probe array on a substrate, having probes capable of specifically making a bond with a target substance possibly contained in said sample as a plurality of mutually independent sopts, wherein a marker is present on a substrate surface such that the positions of said spots can be specified, and detecting the presence of a reaction product of said probe with said target substance in any spot to detect the presence of said target substance in said sample, further comprising the step of specifying the positions of said spots where said reaction product is present on the basis of the positions of the marker on said substrate surface when the presence of said reaction product is detected.
A method of sequencing a single-stranded nucleic acid in a sample comprising the steps of:
- contacting said sample with each spot in a probe array having probes having a complementary sequence to each of expected multiple sequences in said single-stranded nucleic acid as a plurality of mutually independent spots, wherein and where a marker is present on a substrate surface such that the positions of the spots can be specified, and specifying the positions of said spots where a reaction product of said probe with a target substance has been formed on the basis of the positions of said marker on said substrate.
Dependencies are inferred from the claim text.