Novel methods of diagnosing colorectal cancer and/or breast cancer, compositions, and methods of screening for colorectal cancer and/or breast cancer modulators
OwnerDavid Mack, Kurt C. Gish +1inventors
InventorsDavid Mack, Kurt C. Gish, Keith E. Wilson
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Abstract
Described herein are methods that can be used for diagnosis and prognosis of breast and/or colorectal cancer. Also described herein are methods that can be used to screen candidate bioactive agents for the ability to modulate breast and/or colorectal cancer. Additionally, methods and molecular targets (genes and their products) for therapeutic intervention in breast and/or colorectal cancer are described.
Claims31
A method of screening drug candidates comprising:
- a) providing a cell that expresses an expression profile gene encoding CHA4 or fragment thereof;
- b) adding a drug candidate to said cell;
- and
- c) determining the effect of said drug candidate on the expression of said expression profile gene.
A method of screening for a bioactive agent capable of binding to CHA4 or a fragment thereof, said method comprising:
- a) combining said CHA4 or a fragment thereof and a candidate bioactive agent;
- and
- b) determining the binding of said candidate agent to said CHA4 or a fragment thereof.
A method for screening for a bioactive agent capable of modulating the activity of CHA4, said method comprising:
- a) combining CHA4 and a candidate bioactive agent;
- and
- b) determining the effect of said candidate agent on the bioactivity of CHA4.
A method of evaluating the effect of a candidate cancer drug comprising:
- a) administering said drug to a patient;
- b) removing a cell sample from said patient;
- and
- c) determining the expression of a gene encoding CHA4 or fragment thereof.
A method of diagnosing cancer comprising:
- a) determining the expression of a gene CHA4 or a fragment thereof in a first tissue type of a first individual;
- and
- b) comparing said expression of said gene(s) from a second normal tissue type from said first individual or a second unaffected individual;
- wherein a difference in said expression indicates that the first individual has cancer.
An antibody which specifically binds to CHA4 or a fragment thereof.
A method for screening for a bioactive agent capable of interfering with the binding of CHA4 or a fragment thereof and an antibody which binds to CHA4 or fragment thereof, said method comprising:
- a) combining CHA4 or fragment thereof, a candidate bioactive agent and an antibody which binds to CHA4 or fragment thereof;
- and
- b) determining the binding of CHA4 or fragment thereof and said antibody.
A method for inhibiting the activity of CHA4, said method comprising binding an inhibitor to CHA4.
A method of neutralizing the effect of CHA4 or a fragment thereof, comprising contacting an agent specific for said CHA4 or fragment thereof with said CHA4 or fragment thereof in an amount sufficient to effect neutralization.
A method of treating breast cancer and/or colorectal cancer comprising administering to a patient an inhibitor of CHA4.
A method for localizing a therapeutic moiety to breast cancer and/or colorectal cancer tissue comprising exposing said tissue to an antibody to CHA4 or fragment thereof conjugated to said therapeutic moiety.
A method of treating breast cancer or colorectal cancer comprising administering to an individual having said cancer an antibody to CHA4 or fragment thereof conjugated to a therapeutic moiety.
26 The method of claim 24, wherein said therapeutic moiety is a radioisotope.
A method for inhibiting breast cancer or colorectal cancer in a cell, wherein said method comprises administering to a cell a composition comprising antisense molecules to a nucleic acid of FIG. 1.
A biochip comprising one or more nucleic acid segments encoding CHA4 or a fragment thereof, wherein said biochip comprises fewer than 1000 nucleic acid probes.
A method of eliciting an immune response in an individual, said method comprising administering to said individual a composition comprising CHA4 or a fragment thereof.
A method of eliciting an immune response in an individual, said method comprising administering to said individual a composition comprising a nucleic acid encoding CHA4 or a fragment thereof.
A method for determining the prognosis of an individual with breast cancer or colorectal cancer comprising determining the level of CHA4 in a sample, wherein a high level of CHA4 indicates a poor prognosis.
Dependencies are inferred from the claim text.