HCV peptide antigens and methods for the determination of HCV
AssigneeRoche Diagnostics GmbH
InventorsChristoph Seidel, Gertraud Ehrlich-Weinreich, Hubert Bayer, Ursula Henrike Wienhues, Gunther Gerhard Jung, Hans Georg Ihlenfeldt
Life of this patent
Estimating the term…
Abstract
Novel HCV peptide antigens are described representing partial sequences of the C-100-3 and env/core with C-regions. These peptide antigens are suitable for the determination of HCV antibodies as immunogens for the production of antibodies against HCV and as vaccines for the production of vaccines against HCV.
Claims16
HCV peptide antigens with the amino acid sequences SEQ ID NO:
- 1, 2, 11, 12, 15, 16, 22, 23, 29-32 or with partial sequences thereof having a length of at least 4 amino acids.
Combination of HCV peptide antigens composed of
- SEQ ID NO:
- 4, 12, 16
- SEQ ID NO:
- 4, 5, 12 and 16
- SEQ ID NO:
- 3, 4, 5, 12 and 16
- SEQ ID NO:
- 3, 4, 5, 12, 16, 26 and 27
- SEQ ID NO:
- 3, 4, 11, 12, 16 and 26
- SEQ ID NO:
- 3, 4, 12, 16 and 26
- SEQ ID NO:
- 7, 9, 13, 20, 21
- SEQ ID NO:
- 6, 8, 13, 19, 28 or
- SEQ ID NO:
- 25, 30, 31
Method for the determination of HCV antibodies, wherein the sample is incubated with a combination of at least two peptide antigens from the group SEQ ID NO:
- 1, 2, 11, 12, 15, 16, 22, 23, 25, 29-32 or peptide antigens representing partial sequences of these peptide antigens having a length of at least 4 amino acids and the amount of the HCV antibodies bound to the peptide antigen is determined under conditions which enable the formation of an antibody-antigen complex.
Process for the production of antibodies against HCV antigens, wherein a mammal is immunized with a peptide antigen SEQ ID NO:
- 1, 2, 11, 12, 15, 16, 22, 23, 29-32 or a partial sequence thereof which is carrier-bound if desired, polyclonal antibodies are obtained or cells of these animals which produce antibodies are immortalized to form cell lines and monoclonal antibodies are isolated from these cell lines.
Vaccine for the treatment of HCV infections containing a peptide antigen SEQ ID NO:
- 1, 2, 11, 12, 15, 16, 22, 23, 29-32 which is carrier-bound if desired or at least two peptide antigens SEQ ID NO:
- 1-32 or peptide antigens which represent partial sequences of these peptide antigens having a length of at least 4 amino acids as the immunogen, in a pharmacologically effective dose and in a pharmaceutically acceptable formulation.
Process for the production of vaccines using the peptide antigens SEQ ID NO:
- 1, 2, 11, 12, 15, 16, 22, 23, 29-32 or at least two peptide antigens SEQ ID NO:
- 1-32 or peptide antigens representing partial sequences of these peptide antigens having a length of at least 4 amino acids as immunogens.
Dependencies are inferred from the claim text.