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US 2003/0196219 A1Published application
Novel Method for Agrobacterium Preparation for Plant Transformation
AssigneeMonsanto Technology LLC
InventorWanggen Zhang
Life of this patent
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Abstract
The present invention relates to a novel method of preparing Agrobacterium for plant transformation. In particular, the invention relates to storing the Agrobacterium in the cold for some period of time. Surprisingly, this increases transformation efficiency.
Claims10
A method of preparing an Agrobacterium inoculation culture for use in transformation of monocotyledonous plant cells or plant tissue comprising the steps of:
- providing an Agrobacterium inoculation culture at a plant tissue inoculation density;
- storing the Agrobacterium inoculation culture in a cold environment for at least about 12 hours to obtain a chilled Agrobacterium inoculation culture;
- and
- combining the chilled Agrobacterium inoculation culture with plant cells or plant tissue in a manner suitable to achieve transformation of said plant cells or plant tissue.
1 more element
A method of preparing an Agrobacterium inoculation culture for use in the transformation of monocotyledonous plant cells or plant tissue comprising the steps of:
- providing Agrobacterium inoculation culture at a plant tissue inoculation density;
- storing the Agrobacterium inoculation culture at a temperature of about 4° C. for a time period from about 4 days to about 7 days to obtain a chilled Agrobacterium inoculation culture;
- and
- combining the chilled Agrobacterium inoculation culture with plant cells or plant tissue in a manner suitable to achieve transformation of said plant cells or plant tissue.
1 more element
Dependencies are inferred from the claim text.