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AssigneeAbbott Laboratories
InventorsPradip Mukerji, Suzette L. Pereira, Yung-Sheng Huang
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Disclosed are isolated polynucleotides encoding an omega-3 desaturase and a delta-12 desaturase, the enzymes encoded by the isolated polynucleotides, vectors containing the isolated polynucleotides, transgenic hosts that contain the isolated polynucleotides that express the enzymes encoded thereby, methods for producing the desaturase enzymes, and method of using the enzymes to make polyunsaturated fatty acids. The isolated polynucleotides are derived from a fungus, Saprolegnia diclina (ATCC 56851). In particular, omega-3-desaturase may be utilized, for example, in the conversion of arachidonic acid (AA) to eicosapentaenoic acid (EPA). Delta-12 desaturase may be used, for example, in the conversion of oleic acid (OA) to linoleic (LA). EPA or polyunsaturated fatty acids produced therefrom may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
An isolated nucleotide acid sequence or fragment thereof comprising or complementary to a nucleotide sequence encoding a polypeptide having desaturase activity, wherein the amino acid sequence of said polypeptide has at least 50% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:26 and SEQ ID NO:42.
An isolated nucleotide sequence or fragment thereof comprising or complementary to at least 50% of the nucleotide sequence selected from the group consisting of SEQ ID NO:25 and SEQ ID NO:41.
A purified polypeptide that desaturates a polyunsaturated fatty acid substrate at an omega-3 carbon of said substrate and has at least 50% amino acid identity to an amino acid sequence comprising SEQ ID NO:
A purified polypeptide that desaturates a polyunsaturated fatty acid substrate at a delta-12 carbon of said substrate and has at least 50% amino acid identity to SEQ ID NO:
A method of producing a desaturase comprising the steps of:
A vector comprising:
A method for producing a polyunsaturated fatty acid comprising the steps of:
A method of producing a polyunsaturated fatty acid comprising exposing a fatty acid substrate to a polypeptide having at least 50% amino acid identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
Dependencies are inferred from the claim text.