Bioactive compound and its isolation and method of treatment for lipoxygenase inhibition and as free radical scavenging agent

A process for the isolation of bioactive compound having a molecular formula C13H-15NO5, said process comprising the steps of:

• (a) isolating the strain CFR-W-105 from Aspergillus niger V. Teigh from honey bee wax;
• (b) propagating the strain obtained from step(a) on a Potato Dextrose Agar medium and incubating for 4 days at 30° C.;
• (c) inoculating with a slant of step (b) into seed liquid medium contained in Erlenmeyer flask;
• (d) incubating the liquid medium of step (c) in Erlenmeyer flask at 30° C. on a rotary shaker at 250 rpm to obtain the seed culture;
• (e) transferring the culture of step (d) into Erleumeyer flasks containing wheat bran, mineral acid, sulfates and incubated for 5 days at 30° C. to obtain fermented wheat bran;
• (f) treating the fermented wheat bran of step (e) with an organic solvent for two hours to obtain an organic solvent extract;
• (g) separating the organic solvent extract of step(f) from the wheat bran by cheese cloth filtration;
• (h) drying the organic layer of step (g) over anhydrous sodium sulfate and concentrating under reduced pressure to obtain a solid;
• (i) suspending the solid of step (h) in an organic solvent and centrifuging to obtain a residue;
• (j) drying the residue of step (i) to obtain an orange solid;
• (k) dissolving the solid of step(j) in an alcoholic solvent;
• (l) treating the solution of step(k) with active charcoal, filtering;
• and
• (m) concentrating the filtrate under reduced pressure to obtain compound having a molecular formula C13H15NO5 as yellow amorphous powder.