US 2003/0195163 A1 · Polynucleotides encoding three novel human cell surface proteins with leucine rich repeats and immunologobulin folds, BGS2, 3, and 4 and variants thereof

Abstract

The present invention provides novel polynucleotides encoding BGS-2, 3, and 4 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel BGS-2, 3, and 4 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:
1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID,NO:
1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:
2 or the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
1, having cell adhesion protein activity;
(f) an isolated polynucleotide comprising nucleotides 4 to 1776 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 592 of SEQ ID NO:
2 minus the start codon;
(g) an isolated polynucleotide comprising nucleotides 1 to 1776 of SEQ ID NO:
1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 592 of SEQ ID NO:
2 including the start codon;
(h) a polynucleotide comprising nucleotides 71 to 1776 of SEQ ID NO:
1, wherein said nucleotides encodes the mature polypeptide of SEQ ID NO:
2 (i) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
1;
a polynucleotide fragment of SEQ ID NO:
3 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
3;
(k) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
4 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
3;
(l) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
4 or a polypeptide domain encoded by the CDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
3;
(m) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
4 or a polypeptide epitope encoded by the CDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
3;
(n) a polynucleotide encoding a polypeptide of SEQ ID NO:
4 or the CDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
3, having cell adhesion protein activity;
(o) an isolated polynucleotide comprising nucleotides 4 to 1818 of SEQ ID NO:
3, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 606 of SEQ ID NO:
4 minus the start codon;
(p) an isolated polynucleotide comprising nucleotides 1 to 1818 of SEQ ID NO:
3, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 606 of SEQ ID NO:
4 including the start codon;
(q) a polynucleotide comprising nucleotides 82 to 1818 of SEQ ID NO:
3, wherein said nucleotides encodes the mature polypeptide of SEQ ID NO:
4
(r) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
3;
(s) a polynucleotide fragment of SEQ ID NO:
5 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
5;
(t) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
6 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
5;
(u) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
6 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
5;
(v) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
6 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
5;
(w)a polynucleotide encoding a polypeptide of SEQ ID NO:
6 or the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
5, having cell adhesion protein activity;
(x) an isolated polynucleotide comprising nucleotides 4 to 1770 of SEQ ID NO:
5, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 590 of SEQ ID NO:
6 minus the start codon;
(y) an isolated polynucleotide comprising nucleotides 1 to 1770 of SEQ ID NO:
5, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 590 of SEQ ID NO:
6 including the start codon;
(z) a polynucleotide comprising nucleotides 88 to 1770 of SEQ ID NO:
5, wherein said nucleotides encodes the mature polypeptide of SEQ ID NO:
6
(aa) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
5;
(bb) a polynucleotide fragment of SEQ ID NO:
153 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
153;
(cc) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
154 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
153;
(dd) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
154 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
153;
(ee) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
154 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
153;
(ff) a polynucleotide encoding a polypeptide of SEQ ID NO:
154 or the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:
153, having cell adhesion protein activity;
(gg) an isolated polynucleotide comprising nucleotides 506 to 2281 of SEQ ID NO:
153, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 593 of SEQ ID NO:
154 minus the start codon;
(hh) an isolated polynucleotide comprising nucleotides 503 to 2281 of SEQ ID NO:
153, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 593 of SEQ ID NO:
154 including the start codon;
(ii) a polynucleotide comprising nucleotides 590 to 2281 of SEQ ID NO:
153, wherein said nucleotides encodes the mature polypeptide of SEQ ID NO:
154
(jj) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:
153;
and
(kk) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-jj), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

122 more elements

Polynucleotides encoding three novel human cell surface proteins with leucine rich repeats and immunologobulin folds, BGS2, 3, and 4 and variants thereof

Life of this patent

Polynucleotides encoding three novel human cell surface proteins with leucine rich repeats and immunologobulin folds, BGS2, 3, and 4 and variants thereof

Life of this patent

Abstract

Claims24