BAP-1: methods of assaying for cell-cycle modulators
AssigneeRigel Pharmaceuticals, Inc.
InventorsYasumichi Hitoshi, Yonchu Jenkins
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Abstract
The present invention relates to regulation of cellular proliferation. More particularly, the present invention is directed to nucleic acids encoding BAP-1, which is involved in modulation of cell cycle arrest. The invention further relates to methods for identifying and using agents, including small molecule chemical compositions, antibodies, peptides, cyclic peptides, nucleic acids, RNAi, antisense nucleic acids, and ribozymes, that modulate cell cycle arrest via modulation of BAP-1; as well as to the use of expression profiles and compositions in diagnosis and therapy related to cell cycle regulation and modulation of cellular proliferation, e.g., for treatment of cancer and other diseases of cellular proliferation.
Claims36
A method for identifying a compound that modulates cell cycle arrest, the method comprising the steps of:
- (i) contacting a cell comprising an BAP-1 polypeptide or fragment thereof with the compound, wherein the BAP-1 polypeptide is encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid encoding a polypeptide having an amino acid sequence of SEQ ID NO:2;
- and
- (ii) determining the chemical or phenotypic effect of the compound upon the cell comprising the BAP-1 polypeptide or fragment thereof, thereby identifying a compound that modulates cell cycle arrest.
A method for identifying a compound that modulates cell cycle arrest, the method comprising the steps of:
- (i) contacting the compound with an BAP-1 polypeptide or a fragment thereof, wherein the BAP-1 polypeptide or fragment thereof is encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid encoded by a polypeptide comprising an amino acid sequence of SEQ ID NO:2;
- (ii) determining the physical effect of the compound upon the BAP-1 polypeptide;
- and
- (iii) determining the chemical or phenotypic effect of the compound upon a cell comprising an BAP-1 polypeptide or fragment thereof, thereby identifying a compound that modulates cell cycle arrest.
The method of claim 24, wherein the physical effect is measured by assaying form BCRA-1 binding to BAP-1.
A method of modulating cell cycle arrests in a subject, the method comprising the step of administering to the subject a therapeutically effective amount of a BAP-1 polypeptide, wherein the polypeptide is encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid encoding a polypeptide having an amino acid sequence of SEQ ID NO:2.
A method of modulating cell cycle arrest in a subject, the method comprising the step of administering to the subject a therapeutically effective amount of a nucleic acid encoding a BAP-1 polypeptide, wherein the nucleic acid hybridizes under stringent conditions to a nucleic acid encoding a polypeptide having an amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.