Peptides for detection of antibody to Ehrlichia equi
OwnerThomas Patrick O'Connor JR.inventor
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods for the detection and quantification of Ehrlichia equi antibodies and antibody fragments.
Claims25
A composition of matter consisting essentially of an isolated polypeptide shown in SEQ ID NO:1 or SEQ ID NO:2.
A composition of matter consisting essentially of an isolated polypeptide fragment of at least about 5 contiguous amino acids of SEQ ID NO:1 or SEQ ID NO:2.
A method of detecting antibodies specific for Ehrlichia equi comprising:
- (a) contacting a polypeptide shown in SEQ ID NO:1, SEQ ID NO:2 or both SEQ ID NO:1 and SEQ ID NO:2 with a test sample suspected of comprising antibodies specific for Ehrlichia equi, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for Ehrlichia equi are present in the test sample.
An article of manufacture comprising packaging material and, contained within the packaging material, a polypeptide shown in SEQ ID NO:1, SEQ ID NO:2, or both polypeptides shown in SEQ ID NO:1 and SEQ H) NO:2.
A method of diagnosing an Ehrlichia equi infection in a mammal comprising:
- (a) obtaining a biological sample from a mammal suspected of having an Ehrlichia equi infection;
- (b) contacting a polypeptide shown in SEQ ID NO:1, SEQ ID NO:2, or both SEQ ID NO:1 and SEQ ID NO:2, with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the mammal has an Ehrlichia equi infection.
An antibody that specifically binds to at least one epitope of an Ehrlichia equi polypeptide, wherein said polypeptide is SEQ ID NO:1 or SEQ ID NO:2.
Dependencies are inferred from the claim text.