Method for identifying modulators of ion channels
AssigneeOrtho-McNeil Pharmaceutical, Inc.
InventorsAdrienne Dubin, Sandra Chaplan, Sean Brown, Edward Kaftan
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Abstract
The present invention provides methods to manipulate differentiation of a neuroblastoma cell line (IMR-32) such that predominant Nav expression is either Nav1.3 in IMR-32 cells exposed to retinoic acid or Nav1.7 in cells grown under non-differentiating conditions. The cells of the present invention are useful for the discovery of new compounds that modulate the function of either Nav1.3 and/or Nav1.7.
Claims12
A method for inducing expression of Nav 1.3 ion channels from IMR-32 cells comprising culturing the IMR-32 cells with a sufficient amount of a differentiating agent for a sufficient period of time for the IMR-32 cells to produce the Nav 1.3 ion channels.
A cell line expressing Nav 1.7 ion channels wherein said cell line is IMR-32.
Dependencies are inferred from the claim text.