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US 2003/0194717 A1Published application
Mass labels
AssigneeElectrophoretics Limited
InventorsGunter Schmidt, Andrew Hugin Thompson, Robert Alexander Walker Johnstone
Life of this patent
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AssigneeElectrophoretics Limited
InventorsGunter Schmidt, Andrew Hugin Thompson, Robert Alexander Walker Johnstone
Estimating the term…
Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker to a mass normalisation moiety, the mass marker moiety being fragmentation resistant, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectrometry.
A set of tow or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein the set comprises a group of labels having a mass marker moiety of a common mass or the set comprises of a group of labels having a common aggregate mass, and wherein the in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group such that all of the mass labels in the set are distinguishable from each other by mass spectrometry.
A set of mass labels according to any preceding claim, wherein the cleavable linker attaching the mass marker moiety to the mass normalisation moiety is a linker cleavable by collision.
A set of mass labels according to any preceding claim, wherein the mass marker moiety and/or the mass normalisation moiety comprises a fragmentation resistant group.
A set of mass labels according to any preceding claim, wherein the mass marker moiety comprises a pre-ionised group.
A method according to claim 59 or claim 60, which method comprises generating Sanger ladder nucleic acid fragments from a plurality of nucleic acid templates present in the same reaction zone, and for each nucleic acid fragment produced identifying the length of the fragment, the identity of the template from which the fragment is derived and the terminating base of the fragment, wherein prior to generating the fragments, a labelled primer nucleotide or oligonucleotide is hybridised to each template, the label on each primer being specific to the template to which that primer hybridises to allow identification of the template.
Dependencies are inferred from the claim text.