Reversible association of nucleic acid with a carboxylated substrate
AssigneeCapitol Genomix, Inc.
InventorsIndira Carey, Teri Heiland, Anna Smith, Jill Ray
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Abstract
The present invention is directed to methods and compositions wherein nucleic acids are associated with a solid phase that comprises a carboxylated substrate. In specific embodiments, precipitation of the nucleic acids occurs in the absence of salt.
Claims48
A method of isolating nucleic acid, comprising
- contacting a composition comprising nucleic acid with an effective amount of glycogen to facilitate precipitation of nucleic acid and ethanol in the presence of a carboxylated paramagnetic bead, wherein said contact results in the association of the nucleic acid with the carboxylated paramagnetic bead.
A method for isolating nucleic acid, comprising
- contacting a composition comprising nucleic acid with an effective amount of glycogen to facilitate precipitation of nucleic acid and ethanol in the presence of a carboxylated substrate, wherein said contact results in the association of the nucleic acid with the carboxylated substrate.
A kit for nucleic acid isolation, comprising in a suitable containing means:
- a carboxylated substrate;
- and
- a binding solution containing glycogen.
A composition for reversibly binding nucleic acid to a carboxylated substrate, comprising:
- ethanol;
- a nucleic acid;
- an effective amount of glycogen to facilitate precipitation of nucleic acid;
- and
- a carboxylated substrate.
An instrument for isolating nucleic acid, comprising:
- a means for contacting a composition comprising nucleic acid with an effective amount of glycogen to facilitate precipitation of nucleic acid and ethanol in the presence of a carboxylated substrate, wherein said contact results in the association of the nucleic acid with the carboxylated substrate.
An instrument for isolating nucleic acid comprising:
- means for containing a nucleic acid sample;
- means for adding ethanol to the nucleic acid sample means for adding glycogen to the nucleic acid sample in an amount effective to cause precipitation of the nucleic acid;
- a carboxylated substrate in contact with the nucleic acid sample.
Dependencies are inferred from the claim text.