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US 2003/0194706 A1Published application
Selective elution of immobilized multiplexed primer extension products
AssigneeApplera Corporation
InventorMaxim G. Brevnov
Life of this patent
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Abstract
The present invention provides methods and kits for the sequential elution of families of extension products from one or more substrates. The methods and kits can be used, for example, to separate families of primer extension products generated by multiplex sequencing reactions or by other multiplex primer extension reactions.
Claims18
A method of isolating a plurality of families of primer extension products from a capture substrate comprising the steps of:
- (a) selectively eluting a first family of primer extension products captured on the capture substrate, and
- (b) selectively eluting a second family of primer extension products captured on the capture substrate.
A kit for isolating a plurality of families primer extension products comprising:
- (a) a plurality of prey moieties,
- (b) a capture substrate, and
- (c) a plurality of specific elution compounds.
Dependencies are inferred from the claim text.