Methods of using unnatural nucleobases for decoding
OwnerGary P. Schrothinventor
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Abstract
The present invention provides methods and compositions useful for coding and decoding complex mixtures of test units. These methods and compositions use coding and decoding oligonucleotides that comprise standard nucleobases and also non-standard nucleobases that selectively base pair with other non-standard nucleobases. These non-standard nucleobases nucleobases display little or no selective base pairing with standard nucleobases. The use of the non-standard nucleobases increases the diversity of the coding oligonucleotides and reduces the cross-reactivity of the coding and/or decoding oligonucleotides with other molecules.
Claims20
A method of identifying a coded test unit in a plurality of coded test units comprising the step of:
- contacting the coded test unit with a decoding oligonucleotide comprising an orthogonal nucleobase under conditions in which the decoding oligonucleotide produces a detectable hybridization signal sufficient to distinguish the coded test unit from the remainder of the plurality of coded test units.
A coded substrate comprising a test moiety and a coding oligonucleotide, said coding oligonucleotide comprising an orthogonal nucleobase.
Dependencies are inferred from the claim text.