Method of prevention of lysosomal leakage in eukaryotic cells by using 6-methyluracil based water-soluble compounds and method of producing thereof

A method for prevention of lysosomal leakage in eukaryotic cells including a step of exposing said cells to a 6-methyluracil based water-soluble compound.

A method of providing cytoprotection by prevention of lysosomal leakage in eukaryotic cells exposed to a sub-optimal condition including a step of exposing said cells to a 6-methyluracil based water-soluble compound prior or during said exposure.

A method for improving eukaryotic cell survival during long-term cryogenic storage including a step of exposing said cells to a composition comprising a 6-methyluracil based water soluble compound prior, during, and after cryo-suspension, said composition dissolved in a biocompatible water solution in a concentration from about 10 to about 500 micrograms per milliliter.

A method for extending a life span of primer cell cultures including exposure thereof to a 6-methyluracil based water soluble compound dissolved in a biocompatible water solution in concentration from about 1 to about 1000 micrograms per milliliter.

A method of preservation of a donor organ for organ transplantation including a step of exposing said organ to a 6-methyluracil based water-soluble compound dissolved in a biocompatible water solution in concentration from about 1 to about 1000 micrograms per milliliter.

A method of yeast lyophilization including exposure of said yeast prior to lyophilization to a 6-methyluracil based water-soluble compound dissolved in a biocompatible water solution in concentration from about 1 to about 1000 micrograms per milliliter.

A pharmaceutical composition for treatment of excessive or inappropriate apoptosis, said composition comprising a 6-methyluracil based water-soluble compound and a pharmaceutically acceptable carrier or diluent, said compound dissolved in said carrier in concentration from about 1 to about 1000 micrograms per milliliter.

A method of blocking excess or inappropriate apoptosis in a mammal or a human in need of such treatment, said method comprising administering to said mammal or human an effective amount of 6-methyluracil based water soluble compound, or pharmaceutically acceptable solution thereof.

A method of reducing proteolytic decay and general preservation of a food article including exposing said article to a 6-methyluracil based water-soluble compound during at least a portion of a storage period.

The method as in clam 34, wherein said compound is added to the salting solution in concentration of about 100 mg per kilogram of said fish.

A method of protecting eukaryotic cells against radiation comprising a step of administering to said cells of a 6-methyluracil based water-soluble compound in a pharmaceutically acceptable form.

A method of producing a complex of 2,4-dihydroxy-6-methylpyrimidine with N-methyl-D-glucamine consisting essentially of dissolving 2,4-dihydroxy-6-methylpyrimidine in an aqueous solution of equimolar amount of N-methyl-d-glucamine at a temperature from about 60 to about 70 degrees Celsius, followed by isolating of the desired product from the solution.