Methods for making plants tolerant to glyphosate and compositions thereof
AssigneeMonsanto Technology LLC
InventorsScott R. Baerson, Damian J. Rodriguez, Gregory R. Heck
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Abstract
The methods and materials disclosed herein are directed to glyphosate herbicide tolerance in plants. In particular, the isolation of a glyphosate resistant EPSP synthase coding sequence and its regulatory elements from Eleusine indica. The coding sequence and regulatory sequences are useful to genetically engineer plants for tolerance to glyphosate herbicide.
Claims26
A DNA molecule that encodes a naturally occurring glyphosate resistant EPSPS enzyme derived from a glyphosate tolerant plant, wherein the glyphosate resistant EPSPS enzyme has a Km for phosphoenolpyruvate (PEP) of less than 10 μM.
A DNA molecule that encodes a naturally occurring glyphosate resistant EPSPS enzyme of SEQ ID NO:
- 7.
A recombinant DNA molecule comprising:
- a promoter that functions in plant cells to cause the production of an RNA sequence, operably linked to;
- a structural DNA sequence that causes the production of an RNA sequence that encodes an EPSPS enzyme comprising the sequence of SEQ ID NO:
- 7, operably linked to;
- a 3′ non-translated region that functions in plant cells to cause the addition of polyadenyl nucleotides to the 3′end of the RNA sequence, wherein the promoter is heterologous with respect to the structural DNA sequence and selected so as to cause sufficient expression of the polypeptide to enhance the glyphosate tolerance of a transgenic plant cell containing said recombinant DNA molecule.
A method of producing glyphosate tolerant plants comprising the steps of:
- a) inserting into the genome of a plant cell a recombinant DNA molecule comprising:
- a promoter that functions in plant cells to cause the production of a RNA sequence, operably linked to;
- a structural DNA sequence that caused the production of a RNA sequence that encodes an EPSPS enzyme having the sequence of SEQ ID NO:
- 7, operably linked to;
- a 3′ non-translated region that functions in plant cells to cause the addition of polyadenyl nucleotides the 3′end of the RNA sequence;
- where the promoter is heterologous with respect to the structural DNA sequence and adapted to cause sufficient expression of the polypeptide to enhance the glyphosate tolerance of a plant cell transformed with the DNA molecule;
- b) obtaining a transformed plant cell;
- and
- c) regenerating from the transformed plant cell a genetically transformed plant which has increased tolerance to glyphosate herbicide.
A recombinant DNA molecule comprising:
- a promoter that functions in plant cells to cause the production of an RNA sequence, operably linked to;
- a structural DNA sequence that causes the production of an RNA sequence which encodes an EPSPS enzyme having the sequence of SEQ ID NO:
- 7, operably linked to;
- a 3′ non-translated region that functions in plant cells to cause the addition of polyadenyl nucleotides the 3′ end of the RNA sequence, wherein the promoter is homologous with respect to the structural DNA sequence.
Dependencies are inferred from the claim text.