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AssigneeSapporo Breweries Limited
InventorsYukio Okada, Kazutoshi Ito
Estimating the term…
An isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. Hops are dioecious, and only female plants bear cones, the lupulin glands of which contain secondary metabolic products which provide bitterness and flavor to beer. These secondary metabolic products contain some pharmacologically effective compounds. In order to breed a more useful cultivar of hops by manipulating the constituent of such useful secondary metabolic products relying on genetic engineering techniques, this invention provides an isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. By using this nucleic acid, it is possible to develop a novel method for breeding hops with transformation techniques and molecular selection techniques. Furthermore, the present invention also provides a nucleic acid comprising the regulatory region for specifically expressing genes in lupulin glands. This nucleic acid can be used also for hop breeding.
An isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands or a portion thereof.
An isolated and purified nucleic acid comprising a regulatory sequence for the specific expression of genes in hop lupulin glands.
A nucleic acid primer comprising the DNA sequence of SEQ ID NO:
A kit for detecting regulatory sequences for the specific expression of lupulin-specific expression genes, comprising at least one nucleic acid having the base sequence of SEQ ID NOs:
Dependencies are inferred from the claim text.
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Transferring groups other than amino-acyl groups: naringenin-chalcone synthase , i.e. chalcone synthase
Methods for controlling, regulating or enhancing expression of transgenes in plant cells: developmentally regulated expression systems, tissue,…
Reproductive tissue-specific promoters: female-specific, e.g. pistil, ovule
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: involving biosynthetic or metabolic…
Term end not estimated
1. Field of the Invention
The present invention relates to nucleic acids comprising a gene specifically expressed in lupulin glands of hops and to regulatory sequences thereof.
2. Discussion of the Background
FIG. 1: diagram representing the procedure of inverse PCR in the case of isolation of the regulatory sequence in the lupulin-specific gene.
FIG. 2: results of Northern blot analysis of RNAs which have been recovered from lupulin-rich fraction and lupulin-poor fraction and electrophoresed using a lupulin-specific gene as the probe. The analytical results indicate the specificity of lupulin-specific gene expression in lupulin glands.
By the above-described expression “specifically expressed” or “specifically functions,” it is meant that the genes are expressed or functioning not only in lupulin glands alone but also doing so more in these glands as compared to other organs. That is, whether the genes are expressed or functioning “specifically” in lupulin glands or not can be determined by their expression amount and function intensity in lupulin glands compared with other organs.
Furthermore, by the expression “specifically expressed” or “specifically functions” it is not only meant that the genes are as specific as defined above throughout the entire developing period, etc. but also that the expression and function of the genes are more highly elevated by the specific developing period or external influences compared with other organs.
The above-described nucleic acids comprise both DNA and RNA Also, the type of “genes” coded in the above-described nucleic acids includes any types such as genomic DNA, cDNA and mRNA.