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AssigneesCouncil of Scientific and Industrial Research, Indian Council of Agricultural Research
InventorsVinay Mahajan, Subrahamnium Nagarajan, Vishnu Hari Deshpande, Ramesh Ganesh Kelkar, Rajgopal Jagannath Lahoti, Sadyandy Ramlingam, Vivek Jagannathrao Bulbule
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The present invention relates to a novel composition comprising a mixture of potassium 1-[4-chlorophenyl]-1, 4 dihydro-6-methyl-4-oxopyridazine-3carboxylate is in the range of from 1 ppm to 300 ppm and potassium 1-[4-fluoro-3-chlorophenyl]-1, 4 dihydro-6-methyl-4-oxopyridazine-3-carboxylate and a non-ionic surfactant for hybrid seed production. The composition of the invention enhances functional male sterility with improved female fertility facilitating commercial production of hybrid seed of wheat and other crops, cereals in particular, across a range of environments.
A composition for hybrid seed production, comprising a mixture of potassium 1-[4-chlorophenyl]-1,4 dihydro-6-methyl-4-oxopyridazine-3-carboxylate of the formula 1
A process for the preparation of a composition comprising a mixture of potassium 1-[4-chlorophenyl]-1,4 dihydro-6-methyl-4-oxopyridazine-3-carboxylate of the formula 1
Use composition comprising a mixture of compounds of formula 1 and 2 in an non-ionic surfactant for hybrid seed production in a plant.
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Having rings with two nitrogen atoms as the only ring hetero atoms: 1,2-Diazines; Hydrogenated 1,2-diazines
Preservation of bodies of humans or animals or plants or parts thereof
Term end not estimated
The present invention relates to a composition for hybrid seed production. More particularly the present invention also relates to a composition useful for improving female fertility and seed setting in plants. The composition of the present invention is useful for production of hybrid seed in crop plants such as wheat.
Capability of certain classes of chemicals viz. pyridazines, 4-pyridionones, pyrrole carboxylates, 5-chlorocaboxylic acids to effect such changes is know in the literature. Certain compounds designated as DPX 3778, Hybrex, LY 195259, Mendok, RH 2956, RH 4667, RH 5148 and WL 84811 are few of the examples of such compounds. (Pickett A A 1993. In ‘Advances in plant Breeding’15, Paul Parey Scientific Publisher, Berlin, pp 122 -132). However, these chemicals also affect female sterility resulting in lower seed setting and seed germination.
The prior art compositions/compounds suffer from several disadvantages in use such as resulting in some growth inhibition,, severe plant damage, small seeds, inhibition of growth and flowering, leaf burn, shorter plant, etc. In view of the above limitations it is necessary to provide chemicals and their combinations, which may provide foolproof male sterility with better female fertility for commercial seed production of hybrid seed. Although the study and the research was carried out on wheat in the present description, the involved compositions are not only applicable to the wheat plant but are also to other angiosperm crops as rice, maize, jowar, bajra, etc.
The effects of the composition with varied concentration of compound 1 and 2 were evaluated for two crops years. The aqueous solution of the compositions were sprayed on the wheat plants when the Spike length of 7-12 mm. i.e. growth of 20-22 of Zadoks scale, reached between 40-50 days after germination. The results are summarized in Table -3. 3 TABLE 3 Effect of spray of chemicals in combinations over individual molecules Composition Seeds/ Female Seed Germination Seed Seed with concentration spike sterility grade (%) produced produced Treatment of 1 & 2 (ppm) unbagged (%) (A-E* & %) Expected # (g) (% to check) 1 800 + 200 37.8 15.0 B80C20 70.0 28.0 66.2 2 700 + 300 40.7 8.5 A80B20 87.00 37.4 88.4 3 600 + 400 16.2 63.6 B80C20 70.0 12.0 — 4 500 + 500 22.5 49.4 A30C70 62.0 14.3 — 5 400 + 600 10.7 76.0 B20C80 55.0 6.0 — 6 300 + 700 7.6 82.9 A80B20 87.0 7.0 — 7 200+ 800 13.5 44.2 A80b20 87.0 12.4 — 8 1000+ 0 31.0 30.3 A20b80 78.0 25.7 60.7 9 0 + 1000 24.8 46.1 A90b10 88.5 23.2 54.8 Check 44.5 — A100 90.0 42.3 100.0 Note* A: Normal seed and E: Highly shriveled seed # To calculate expected seed germination A, B, C, D, and E (visual scoring) equated as 0.90, 0.75, 0.50, 0.25 and 0.05 respectively.
In a future of the present invention the calculation of male/female sterility is based on number of seeds and is done as per formula given below: 1 Male Sterility ( % ) = ( Untreated Control - Bagged Spikes ) × 100 Untreated Control Female Sterility ( % ) = ( Untreated Control - Bagged Spikes ) × 100 Untreated Control
(Mahajan V, Singh K, Kelkar R. G. 2000. In “Wheat Research Needs Beyond 2000AD (Eds. Nagajana S, Singh G and Tyagi B S) Narosa Publishing House, New Delhi pp 101-106.)