Methods of regulating cytokine signalling

A method for identifying a modulator of interaction between a SOCS molecule or part, fragment, or derivative, or homologue or analogue thereof and a receptor for a cytokine or related molecule or part, fragment derivative or homologue or analogue thereof, said method comprising:—

• immobilizing one of said SOCS molecules or receptor or their parts, fragments, derivatives, homologues or analogues to a solid support optionally via binding partners wherein one of said binding partners is attached or otherwise anchored to said solid support and another of said binding partners is linked to said SOCS or receptor molecules;
• contacting said immobilized SOCS or receptor molecule with the other of receptor or SOCS molecule in the presence of a potential agonist or antagonist;
• and
• measuring qualitatively or quantitatively a change in signal emission indicative of enhanced or diminished binding between said SOCS and receptor or their parts, fragments, derivatives, homologues or analogues.

A method for identifying a modulator of interaction between a SOCS molecule or part, fragment, or derivative, or homologue or analogue thereof and a receptor for a cytokine or related molecule or part, fragment derivative or homologue or analogue thereof, said method comprising:—

• immobilizing said receptor or its parts, fragments, derivatives, homologues or analogues to a solid support optionally via binding partners wherein one of said binding partners is attached or other vise anchored to said solid support and another of said binding partners is linked to said receptor molecule;
• contacting said immobilized receptor molecule with said SOCS molecule or its parts, fragments, derivatives, homologues or analogues in the presence of a potential agonist or antagonist;
• and
• measuring qualitatively or quantitatively a change in signal emission indicative of enhanced or diminished binding between said SOCS and receptor or their parts, fragments, derivatives, homologues or analogues.

A method for identifying a modulator of interaction between a SOCS molecule or part fragment, or derivative, or homologue or analogue thereof and a receptor for a cytokine or related molecule or part, fragment derivative or homologue or analogue thereof, said method comprising:—

• immobilizing one of said SOCS molecules or receptor or their parts, fragments, derivatives, homologues or analogues to a solid support optionally via binding partners comprising biotin and streptavidin wherein one of said biotin or streptavidin is attached or otherwise anchored to said solid support and the another of said biotin or streptavidin is linked to said SOCS or receptor molecule;
• contacting said immobilized SOCS or receptor molecule with the other of receptor or SOCS molecule in the presence of a potential agonist or antagonist;
• and
• measuring qualitatively or quantitatively a change in signal emission indicative of enhanced or diminished binding between said SOCS and receptor or their parts, fragments, derivatives, homologues or analogues.

A method of identifying a modulator of interaction between a SOCS molecule or part, fragment, or derivative, or homologue or analogue thereof and a receptor for a cytokine or related molecule or part, fragment derivative or homologue or analogue thereof, said method comprising:

• immobilizing said receptor or its parts, fragments, derivatives, homologues or analogues to a solid support optionally via binding partners comprising biotin and streptavidin wherein streptavidin is attached or otherwise anchored to said solid support and said biotin is linked to said receptor molecule;
• contacting said immobilized receptor molecule with said SOCS molecule or its parts, fragments, derivatives, homologues or analogues in the presence of a potential agonist or antagonist;
• and
• measuring qualitatively or quantitatively a change in signal emission indicative of enhanced or diminished binding between said SOCS and receptor or their parts, fragments, derivatives homologues or analogues.