Modified subtilisins and detergent, compositions containing same

A modified subtilisin 309 or subtilisin 147, comprising a substitution at one or more of the following positions:

• 1, 14, 18, 21, 22, 24, 27, 41, 43, 45, 46, 49, 50, 52, 61, 62, 75, 76, 77, 78, 79, 87, 94, 98, 99, 100, 101, 103, 105, 106, 107, 109, 113, 116, 117, 118, 126, 129, 130, 136, 137, 141, 167, 171, 181, 183, 184, 185, 188, 189, 192, 197, 204, 206, 209, 210, 213, 214, 215, 216, 217, 244, 247, 248, 249, 251, 253, 254, 256, 271, and 272,
• wherein each position corresponds to the position of the amino acid sequence of the mature subtilisin BPN′.